柯萨奇病毒A24变种的血清抗体检测  

Detection of serum antibody of coxsackievirus A24 variant

作　　者：孟瑞华[1] 陈颖[2] 严岚[3] 孙为荣[1] 程红[3] 曹永献[2] 仇宜解[1] 

机构地区：[1]青岛大学医学院附属医院眼科,266003 [2]青岛大学医学院附属医院,266003 [3]首都儿科研究所

出　　处：《中华实验和临床病毒学杂志》1999年第3期284-286,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　观察急性出血性结膜炎（ＡＨＣ）流行后人群中柯萨奇病毒Ａ２４ 变种（ＣＡ２４ ｖ） 感染情况（或状态）。方法　采用中和试验测定人群中血清抗体：血清作１∶１０ 稀释，病毒用１００ＴＣＩＤ５０ 在微量培养板上进行。ＣＡ２４ ｖ 病毒与血清等量混合，３７℃结合１ 小时，接种细胞，观察细胞病变。结果　ＡＨＣ流行后城市居民ＣＡ２４ ｖ 血清中和抗体阳性率为４９－６７％ ，其中１９～２５ 岁年龄组约占６９－４９％ ，统计学上有显著性（ Ｐ＜ ０－０１） 。结论　ＡＨＣ流行后人群的抗体水平，反映人群受ＣＡ２４ ｖ 感染状态、抗体阳性率与年龄有关。Objective To observe the serum antibody in residents after outbreak of acute hemorrhagic conjunctivitis (AHC) caused by coxrackievirus (CA24 v).Methods The serum was diluted in 1∶10 and mixed with 100 TCID50 CA24 v in volume. The same the mixture was incubated 1 hour in 37℃ and then observed for cytopathic changes of the infected HeLa cells.Results The post epidemic antibody positive rate in residents was 49.67% and in 19～25 age group was about 69.49%. It had significant difference statistically ( P <0.01).Conclusion The raising of CA24 v antibody level in residents was relevent with age.May be it was caused by the opporturnities of taking part in social activities in different age groups.

关 键 词：柯萨奇病毒 CA24V 中和抗体 AHC 血清流行病学 

分 类 号：R373.23[医药卫生—病原生物学]