血清CINC浓度在大鼠肝移植后急性排斥反应中的意义  

The role of serum CINC level in acute rejection after liver transplantation in rats.

作　　者：邹小明[1] 袁友萍[1] 杨维良[1] 聂刚[1] 王夫景[1] 姜学海[1] 平田公一 

机构地区：[1]哈尔滨医科大学第二临床医学院普外科,150086 [2]札幌医科大学第一外科

出　　处：《中华器官移植杂志》1999年第4期221-223,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　探讨嗜中性粒细胞趋化因子（ＣＩＮＣ） 在肝移植后再灌注损伤和急性排斥反应发生时的作用。方法　施行了３ 组大鼠原位肝移植。即：（１） ＡＣＩ大鼠ＬＥＷ 大鼠组；（２） ＢＮ 大鼠ＬＥＷ 大鼠组；（３） ＬＥＷ 大鼠ＬＥＷ 大鼠组。分别为高度排斥反应组， 中度排斥反应组和对照组。结果　大鼠肝移植后血清ＣＩＮＣ浓度与正常大鼠相比明显增加（ Ｐ＜ ０ ．００１） ， 同时嗜中性粒细胞总数和天冬氨酸转氨酶（ＡＳＴ） 及丙氨酸转氨酸（ＡＬＴ） 值也增加。急性排斥反应发生时， 随着血清ＣＩＮＣ 浓度的上升， 嗜中性粒细胞总数和ＡＳＴ、ＡＬＴ 值也增加， 其增加幅度与排斥反应强度有关。结论　ＣＩＮＣ不仅参与再灌注损伤， 而且参与急性排斥反应。因此，ＣＩＮＣ可以作为诊断急性排斥反应的指标之一。Objective In order to investigate the role of CINC in reperfusion injury and acute rejection after rat liver transplantation.Methods Three groups of rats underwent the orthotopic liver transplantation: the high rejection group, the middle rejection group and the contol group. The blood was taken from the tail's vein at 12th h, 1st day after liver transplantation. The same operation was repeatedly perfomed every 2 days from then on. The serum CINC level was examined by using ELISA method.Results As compared with control group, the serum CINC level was obviously increased after liver transplantation in the rats ( P < 0.001 ). At the same time, the amount of neutrophils and the level of AST and ALT were also increased with the increase of serum CINC level. As the acute rejection occurred, with the serum CINC level increasing, the amount of neutrophils, AST and ALT were increased also, which was related to the severity of rejection ( P < 0.01 ).Conclusion CINC not only involved in the reperfusion injury, but also acute rejection. So, CINC could be used as one of the indicators for diagnosis of acute rejection.

关 键 词：再灌注损伤 急性 排斥反应 肝脏移植 CINC 

分 类 号：R657.3[医药卫生—外科学]