1-羟基芘与牛血清白蛋白的相互作用  

Interaction between 1-Hydroxypyrene with Bovine Serum Albumin

作　　者：欧阳运富[1] 王永生[2] 李贵英[1] 

机构地区：[1]常州市疾病预防控制中心,常州213022 [2]南华大学公共卫生学院,衡阳421001

出　　处：《理化检验（化学分册）》2011年第3期253-256,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：湖南省自然科学基金资助项目(03JJY3030);常州市自然科学基金资助项目(CS2008209)

摘　　要：采用荧光光谱法和紫外-可见分光光度法研究了1-羟基芘与牛血清白蛋白之间的相互作用。结果表明:1-羟基芘对牛血清白蛋白有较强的荧光猝灭作用。根据由Stern-Volmer方程得到的荧光猝灭常数,可判断由于与1-羟基芘反应而导致牛血清白蛋白的荧光猝灭属于静态猝灭。采用位点结合模型公式和Frster非辐射能量转移理论计算了结合常数、结合位点数、结合距离。从计算得到的热力学参数焓变△H和熵变△S,推断了1-羟基芘与牛血清白蛋白反应的作用力为氢键和范德华力。Mechanism of the interaction between 1-hydroxypyrene （1-HP） and bovine serum albumin （BSA） was studied by fluorospectrometry and UV-VIS spectrophotometry. It was found that significant quenching of fluorescence of BSA appeared by its reaction with 1-HP. As iudged from the fluorescence quenching constants obtained by Stern-Volmer equation, fluorescence quenching of BSA by 1-HP was attributed to static quenching. The binding constant, number of binding sites and binding distance were found by applying the equation of site-binding model and Forster＇s nonradiative energy transfer theory. Values of thermodynamic parameters △H and △S were calculated and based on these results, the binding force of the reaction was due to hydrogen bond and Vander Waals force.

关 键 词：1-羟基芘 牛血清白蛋白 相互作用 荧光光谱法 分光光度法 

分 类 号：O657.32[理学—分析化学]