高效液相色谱法同时测定复方草珊瑚含片中四种活性成分  

Simultaneous HPLC Determination of Four Active Components in Sucking Tablets of "Caoshanhu" Compound

作　　者：李良[1] 郑飞龙 刘芬[3] 

机构地区：[1]武警江西总队医院药剂科,南昌330030 [2]江西省食品药品检验所,南昌330029 [3]南昌大学医学院,南昌330031

出　　处：《理化检验（化学分册）》2011年第3期294-296,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金资助项目(No.20672143)

摘　　要：提出了高效液相色谱法同时测定复方草珊瑚含片中原儿荼酸、3-羟基-4-甲氧基苯甲酸、异嗪皮啶和迷迭香酸的含量。样品经甲醇超声提取,用Waters Sun Fire ODS色谱柱(4.6 mm×150mm,5μm)分离,以不同比例混合的乙腈和磷酸-水(0.1+99.9)溶液为流动相进行梯度洗脱,流量0.8mL·min^(-1),在294 nm波长处用紫外检测器检测。在优化的色谱条件下,4种活性成分可完全分离。原儿茶酸、3-羟基-4-甲氧基苯甲酸、异嗪皮啶和迷迭香酸的线性范围分别为2.508～50.16,18.40～368.0,6.375～127.5,41.04～820.8ng;平均回收率分别为92.8%,93.1%,91.9%,99.6%;相对标准偏差(n=6)均不大于5.0%。HPLC was applied to the determination of 4 activitive components, namely, procatechuic acid, 3- hydroxy-4-methoxy benzoic acid, isofraxindin and rosmarinic acid, in sucking tablets of ＂Caoshanhu＂ compound. The sample was extracted ultrasonically with methanol and the extract was used for the determination. Water SunFire ODS column （4. 6 mm×150 mm, 5 μm） was used as stationary phase, and mixtures of acetonitrile and phosphoric acid （0. 1＋99. 9） solution in various ratios were used as mobile phase in gradient elution with flow-rate of 0. 8 mL·min^-1. Wavelength of 294 nm was chosen for UV-detection of the 4 components. Under optimum chromatographic conditions, linearity ranges were obtained between 2. 508- 50. 16 ng （for procatechuic acid）, 18. 40-368. 0 ng （for 3-hydroxy-4-methoxy benzoic acid）, 6. 375-127. 5 ng （for isofraxindin） and 41. 04- 820. 8 ng （for rosmarinic acid）. Values of average recovery found were 92. 8%, 93. 1%, 91. 9% and 99. 6% respectively. Values of RSDrs （n=6） found were less than or equal to 5. 0%.

关 键 词：高效液相色谱法 复方草珊瑚含片 活性成分 

分 类 号：O652.63[理学—分析化学]