ABA受体RCAR1/PYL9相互作用蛋白质的筛选及初步研究  

Screening and preliminary study of proteins interacting with ABA receptor RCAR1/PYL9

作　　者：李德款[1] 张亮[1] 彭梅芳[1] 胡伟芳[1] 李鹰[1] 蔡黎[1] 李旭锋[1] 杨毅[1] 

机构地区：[1]四川大学生命科学学院与生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2011年第2期486-490,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30470927)

摘　　要：使用拟南芥作为模型,采用酵母双杂交系统筛选出和ABA受体RCAR1/PYL9有相互作用的蛋白质,以更深入的研究RCAR1/PYL9的功能.首先利用反转录PCR方法获得拟南芥的cDNA,然后通过酵母双杂交系统,用拟南芥RCAR1/PYL9作为诱饵蛋白对拟南芥cDNA文库进行筛选,并获得多个阳性克隆.对该菌落进行进一步的鉴定得到与RCAR1/PYL9具有相互作用的蛋白质,这对进一步研究RCAR1/PYL9及其相互作用蛋白质在ABA信号转导途径中的作用及地位奠定了基础.In order to understand the function of ABA receptor RCAR1/PYL9 more clearly, Arabidopsis thialiana was selected as a model to screen proteins which can interact with RCAR1/PYLg. Firstly, cD- NA of Arabidopsis thialiana was used as a bait protein to screen the cDNA library through yeast twohybrid system and several single colonies were assayed. Identification of these colonies to find out the interacting partners of RCAR1/PYL9 provides the foundation for further understanding of the functions and status of these proteins in ABA signal transduction pathway.

关 键 词：RCAR1/PYL9 酵母双杂交系统 CDNA文库 

分 类 号：Q78[生物学—分子生物学]