中国蚕豆种质资源ISSR标记遗传多样性分析  

Genetic Diversity of Chinese Faba Bean (Vcia faba L.) Germplasm Revealed by ISSR Markers

作　　者：王海飞[1] 关建平[1] 马钰[1] 孙雪莲[1] 宗绪晓[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《作物学报》2011年第4期595-602,共8页Acta Agronomica Sinica

基　　金：现代农业产业技术体系建设专项(nycytx-18);农业部作物种质资源保护项目(NB09-2130135-2-09)资助

摘　　要：利用ISSR标记对中国18个省（区）的527份春播区和秋播区蚕豆资源的遗传多样性与亲缘关系进行了分析。结果表明,11个ISSR引物共扩增出278条清晰的条带,其中多态性条带268条（占96%）。不同地理来源蚕豆资源群间的基因多样性指数在0.1267~0.2509之间,平均为0.1716;Shannon指数范围为0.1932~0.3767,平均为0.2608。两指数均以内蒙古资源群最高,云南和甘肃资源群次之,江西资源群体最低。非加权配对算术平均法（UPGMA）的聚类分析和二维主成分分析（PCA）结果表明,中国春播区和秋播区蚕豆资源明显不同,浙江和四川的资源形成独立的组群,明显不同于其他省份,其遗传背景独特,有待进一步研究。中国蚕豆种质资源遗传多样性差异和遗传关系与其生长习性、生态分布及地理来源密切相关。Faba bean is an important grain legume crop with high seed-protien content. It plays a critical role in crop rotation. China is the largest faba bean producer in the world. However, little is known about the genetic potential of indigenous faba bean germplasm from China. The assessment of genetic diversity in faba bean germplasm is important for its utilization in breeding programs. To evaluate genetic diversity and relationship of faba bean germplasm in China, we characterized a total of 527 accessions of faba bean from autumn and spring sowing regions in China using 11 ISSR primers. There were 278 unambiguous bands generated, 268 of which were polymorphic, with the rate of polymorphic bands of 0.96. Nei’s gene diversity and Shannon’s information index ranged from 0.1267–0.2509 and 0.1932–0.3767, respectively, in different geographic groups. The Inner Mongolia group showed the highest value, followed by the Yunnan and Gansu groups. Accessions from Jiangxi showed the lowest diversity. The spring faba bean germplasm was clearly separated from winter faba bean germplasm, based on principal component analysis and UPGMA clustering analysis. Germplasm from Zhejiang and Sichuan was quite distinct from that of other provinces. The results indicated that the genetic relationship and diversity of faba bean in China are closely associated with their growth habit, geographical origin, and ecological distribution.

关 键 词：蚕豆 种质资源 ISSR标记 遗传多样性 

分 类 号：S643.6[农业科学—蔬菜学]