不同体外成熟时间对猪体外成熟卵母细胞孤雌胚胎发育的影响  

Effect of different in vitro maturation durations on development of porcine parthenogenetic activation embryo

作　　者：赵明辉[1] 高阳[2] 梁爽[1] 刘希[1] 尹熙俊[1] 

机构地区：[1]延边大学农学院,吉林延吉133002 [2]珲春市牧业管理局动检站,吉林珲春133300

出　　处：《延边大学农学学报》2011年第1期10-14,共5页Agricultural Science Journal of Yanbian University

基　　金：国家转基因重大专项资金支持项目(2009ZX08006-002B)

摘　　要：对体外成熟33,41,49和57h的猪成熟卵母细胞进行孤雌激活,并对激活后孤雌胚胎的囊胚发育率进行观察.成熟率方面,33h组显著低于41,49和57h组,分别为52.54%,79.19%,93.25%和93.75%(p<0.05);卵裂率方面,41h组显著高于57h组,分别为92.26%和56.17%(p<0.05),其它3组之间没有显著性差异;囊胚率上,41h组显著高于33,49和57h组,分别为41.16%,11.96%,21.20%和26.92%(p<0.05).因此,体外成熟41h最有利于猪孤雌胚胎的囊胚发育.To determine the effect of delayed maturation time on development（cleavage and to the blastocyst） of in vitro maturation porcine oocytes that were electrically activated.At 33 h of maturation in the IVM medium,the percentage（52.54%） of oocytes extruded the 1st polar body significant lower than that at 41,49 and 57 h maturation（79.19%,93.25% and 93.75%）.The proportion of embryos which been matured for 41 h that had cleavaged was significant higher than the proportion at 57 h（92.26% and 57.16,respectively,p〈0.05）.Further,the oocyte matured for 41 h that had formed full blastocysts after electrically activated at a significant greater rate（41.16%,p〈0.05） compared with those matured for 33,49 and 57 h（11.96%,21.20% and 26.92%,respectively）.These results indicated that delayed in vitro maturation time for pig oocytes to 41 h could support the development to blastocyst better in this study.

关 键 词：猪 成熟时间 孤雌激活 胚胎发育 

分 类 号：S813.3[农业科学—畜牧学]