金标法与免疫发光法检测甲胎蛋白在体检中的应用  

Application of detecting AFP by dot immunogold chromatographic assay and fluorescence enzye immunoassay in physical examination.

作　　者：郭景玉[1] 张云林[1] 贺先奇[1] 王京秋[1] 

机构地区：[1]解放军316医院检验科,北京100093

出　　处：《临床和实验医学杂志》2011年第8期621-622,共2页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨金标法和免疫发光法检测甲胎蛋白(AFP)在健康体检中的应用价值。方法对728例健康体检的个人同时应用金标法和免疫发光法测定AFP,并对结果进行分析。结果金标法测定的阳性率为1.51%,免疫发光法测定的阳性率为0.41%,两者比较无显著差异(P>0.05)。结论 对健康体检人群检测AFP宜应先用金标法进行定性,对阳性结果再用免疫发光法进行定量。Objective To investigate the clinical value of using dot immunogold chromatographic assay and fluorescence enzyme immunoassay to detect AFP in physical examination.Methods Dot immunogold chromatographic assay and fluorescence enzyme immunoassay were used to detect AFP in 728 patients and the results were compared.Results There were 11 positive cases（1.51%） in dot immunogold chromatographic assay and 3 positive cases（0.41%） in fluorescence enzyme immunoassay,which showed no statistical differences（P0.05）.Conclusion In physical examination,the AFP should be qualitative detected by dot immunogold chromatographic assay and then the positive cases were quantitative detected by dot immunogold chromatographic assay.

关 键 词：甲胎蛋白 健康体检 金标法 免疫发光法 

分 类 号：R194.3[医药卫生—卫生事业管理]