抗肿瘤抗生素C1027对人结肠癌HCT-8细胞凋亡相关基因表达的影响  

EFFECT OF ANTITUMOR ANTIBIOTIC C1027 ON THE EXPRESSION OF GENES RELATED TO APOPTOSIS IN HCT 8 HUMAN COLON CANCER CELLS

作　　者：宋旭[1] 包明敏[1] 崔大鹏[1] 王真[1] 李电东[1] 

机构地区：[1]中国医学科学院,中国协和医科大学医药生物技术研究所,北京100050

出　　处：《药学学报》1999年第10期734-738,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金;中国医学科学院基金

摘　　要：目的：研究抗肿瘤抗生素Ｃ１０２７ 对人结肠癌ＨＣＴ８ 细胞凋亡相关基因表达的影响。方法：利用ｃＤＮＡ 排列（ｃＤＮＡａｒｒａｙｓ）、Ｎｏｒｔｈｅｒｎ 杂交、ＲＴＰＣＲ 检测技术。结果：用１０ ｎｍｏｌ·Ｌ－ １ Ｃ１０２７ 处理ＨＣＴ８ 细胞８ ｈ 后，利用ｃＤＮＡ 排列检测，发现Ｃ１０２７ 可以促进ＴＲＩＰ，ＴＲＡＦ３ ，ＤＲ４ ，ＭＣＨ６ ，ＭＣＨ４，Ａｐｏ３ ，ＤＲ５，ＡＢＬＬ，ＳＴＡＴ１ 等基因的表达，抑制ｒｈｏＣ的表达。用Ｎｏｒｔｈｅｒｎ 杂交及ＲＴＰＣＲ 也得到了同样结果。结论：Ｃ１０２７ 可能通过调节ＴＮＦ 受体家族有关的凋亡信号传导通路，诱导细胞凋亡。AIM: To study the effcet of C1027 on the expression of genes related to apoptosis in HCT 8 human colon cancer cells. METHODS: HCT 8 human colon cancer cells were treated with C1027(10 nmol·L -1 ) for 8 hours. The effect of C1027 on the expression of genes in HCT 8 cells were detected by cDNA arrays, Northern blot and RT PCR. RESULTS: Hybridization of the entire cDNA populations to Atlas Arrays showed that C1027 up regulated the expression levels of TRAF3, DR4, DR5, MCH4, MCH6, TRIP, Apo 3, ABLL and STAT1 and down regulated the expression levels of rhoC. These changes were confirmed by Northern blot and RT PCR. CONCLUSION: Most of the genes are involved in TNF receptor family related signal transduction. The results indicate that C1027 may induce apoptosis by regulating TNF receptor family related signal transduction.

关 键 词：抗肿瘤抗生素 C1027 人结肠癌 HCT-8细胞 

分 类 号：R979.14[医药卫生—药品] R735.350.5[医药卫生—药学]