UPLC法同时测定冬凌草中5种有效成分的含量  

UPLC simultaneous determination of five effective constituents in Rabdosia rubescens

作　　者：张静[1,2] 袁珂[1] 金永春[1] 刘颖坤[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,临安311300 [2]河南中医学院药学院,郑州450008

出　　处：《药物分析杂志》2011年第4期641-644,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立超高效液相色谱法同时测定冬凌草药材中冬凌草甲素、冬凌草乙素、迷迭香酸、齐墩果酸和熊果酸5个主要有效成分的含量。方法:采用WATERS UPLC色谱系统,ACQUITY BEH Shield RP18色谱柱(2.1 mm×100 mm,1.7μm);流动相为0.1%甲酸甲醇溶液(A)-0.1%甲酸水溶液(B),梯度洗脱(0～9 min,70%A→57%A;9～10 min,57%A→48%A;10～11 min,48%A→48%A;11～14 min,48%A→14%A;14～22 min,14%A→14%A),流速0.2 mL·min-1;检测波长为250 nm(0～12.40min测定冬凌草甲素、冬凌草乙素和迷迭香酸)和210 nm(12.41～22.00 min测定齐墩果酸和熊果酸);柱温23℃。结果:本方法可在22 min内完成5个成分的色谱分析,且各成分色谱峰之间具有良好的分离度;冬凌草甲素、冬凌草乙素、迷迭香酸、齐墩果酸和熊果酸的线性范围分别为0.110～0.990μg(r=0.9997),0.012～0.108μg(r=0.9991),0.030～0.270μg(r=0.9993),0.011～0.099μg(r=0.9991),0.011～0.099μg(r=0.9996);平均加样回收率(n=9)分别为100.9%,95.4%,102.1%,97.1%,99.0%。结论:所建立的超高效液相色谱法简便快速,结果准确可靠,重复性好,可用于冬凌草药材的质量控制与评价。Objective：To establish a UPLC method for simultaneous determination of five effective constituents（oridonin,ponicidin,rosmarinic acid,oleanolic acid and ursolic acid） in different parts of Rabdosia rubescens from various sources.Methods：Five effective constituents were simultaneously determined by UPLC with ACQUITY BEH Shield RP18 column（2.1 mm 100 mm,1.7 μm） by gradient elution（0-9 min,70%A→57%A;9-10 min,57%A→48%A;10-11 min,48%A→48%A;11-14 min,48%A→14%A;14-22 min,14%A→14%A） using 0.1% methanoic acid in methanol（A） and 0.1% methanoic acid（B） as the mobile phase at the flow rate of 0.2 mL·min-1;The detection wavelength was 250 nm（0-12.40 min） for oridonin,ponicidin,rosmarinic acid,and 210 nm（12.41-22 min） for oleanolic acid and ursolic acid with column temperature at 23 ℃.Results：Five effective constituents were separated clearly and respectively in 22 min,the linear ranges of oridonin,ponicidin,rosmarinic acid,oleanolic acid and ursolic acid was 0.110-0.990 μg（r=0.9997）,0.012-0.108 μg（r=0.9991）,0.030-0.270 μg（r=0.9993）,0.011-0.099 μg（r=0.9991）,0.011-0.099 μg（r=0.9996）,respectively;The average recoveries（n=9） were 100.9%,95.4%,102.1%,97.1%,99.0%,respectively.Conclusion：The method is simple,accurate and can be used for quality control and evaluation of Rabdosia rubescens.

关 键 词：超高效液相色谱法 冬凌草 冬凌草甲素 冬凌草乙素 迷迭香酸 齐墩果酸 熊果酸 

分 类 号：R917[医药卫生—药物分析学]