RP-HPLC同时测定黑水缬草药材中缬草烯酸和缬草素含量  

RP-HPLC simultaneous determination of valerenic acid and valepotriate in Valeriana amurensis

作　　者：梁朝锋[1,2] 都晓伟[1] 闫梅[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]芜湖市中医院,芜湖241000

出　　处：《药物分析杂志》2011年第4期671-673,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：黑龙江省杰出青年科学基金资助项目(No.JC200612)

摘　　要：目的:建立同时测定黑水缬草药材中缬草烯酸和缬草素含量的HPLC检测方法。方法:采用Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇(A)-0.5%磷酸溶液(B)为流动相,梯度洗脱(0～5 min,60%A→70%A;5～22 min,70%A→82%A;22～30 min,82%A→82%A;30～35 min,82%A→90%A),流速1 mL·min-1,采用切换波长法(268 nm检测缬草烯酸,255 nm检测缬草素),柱温35℃。结果:缬草烯酸、缬草素质量分别在0.0747～1.1952μg(r=0.9999)和0.0373～0.5971μg(r=0.9998)范围内线性关系良好;平均加样回收率(n=6)分别为99.8%(RSD=1.3%),100.2(RSD=1.4%)。结论:本法操作简便,结果准确,重复性好,为评价黑水缬草药材的质量提供了可靠的分析方法。Objective：To develop an HPLC method for the determination of valerenic acid and valepotriate in Valeriana amurensis.Methods：Diamonsil C18（250 mm×4.6 mm,5 μm） column was adopted,the mobile phase consisted of methanol（A）-0.5% H3PO4 solution（B） with gradient elution（0-5 min,60%A→70%A;5-22 min,70%A→82%A;22-30 min,82%A→82%A;30-35 min,82%A→90%A）at the flow rate of 1 mL·min-1.The detecting wavelength was set at 268 nm for valerenic acid and 255 nm for valepotriate,the column temperature was at 35 ℃.Results：The calibration curve was linear in the range of 0.0747-1.1952 μg for valerenic acid（r=0.9999） and 0.0373-0.5971 μg for valepotriate（r=0.9998）;The average recoveries（n=6） of valerenic acid and valepotriate were 99.8%（RSD=1.3%） and 100.2（RSD=1.4%）,respectively.Conclusion：This HPLC analysis method is simple and reproducible,which can be used for the quality control of Valeriana amurensis.

关 键 词：高效液相色谱 缬草烯酸 缬草素 黑水缬草 

分 类 号：R917[医药卫生—药物分析学]