番木瓜中异硫氰酸苄酯及其前体物质苄基硫代葡萄糖苷的含量分析  

Analysis of benzyl isothiocyanate and its precursor-benzyl glucosinolate in Carica papaya L.

作　　者：李泽友[1,2] 沈文涛[1] 言普[1] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]海南医学院药学系,海口571101

出　　处：《药物分析杂志》2011年第4期678-681,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：海南省自然科学基金资助项目(批准号:309042)

摘　　要：目的:建立番木瓜中抗癌活性物质异硫氰酸苄酯及其前体物质苄基硫代葡萄糖苷的含量测定方法,并测定它们在番木瓜各部位中的含量。方法:苄基硫代葡萄糖苷采用HPLC法,以依利特Hypersil BDS-C18(200 mm×4.6 mm,5μm)为分析柱,用含0.1%三氟乙酸的乙腈-水梯度洗脱,流速1.0 mL·min-1,检测波长214 nm。异硫氰酸苄酯采用GC法,色谱柱为SPB1701石英毛细管柱(30 m×0.32 mm×0.25μm),程序升温,载气为氮气。FID检测器温度280℃,进样口温度250℃。结果:苄基硫代葡萄糖苷在0.91～36.4μg范围内线性关系良好(r=0.9992),异硫氰酸苄酯在0.0174～0.556μg范围内线性关系良好(r=0.9992)。除成熟果肉外,苄基硫代葡萄糖苷在其他部位均能检测到,且以种子中含量最高为4.74 mg·g-1;而异硫氰酸苄酯只在花和种子中检测到,以种子中含量较高为0.605 mg·g-1。结论:所建方法可用于番木瓜中苄基硫代葡萄糖苷和异硫氰酸苄酯的含量测定。Objective：To establish methods for determination of the content of anticancer active material,benzyl isothiocyanate（BITC） and its precursor-benzyl glucosinolate（BG）,and to determine them in different parts of Carica papaya.Methods：HPLC was performed to detect the BG on Hypersil BDS-C18 column（200 mm×4.6 mm,5 μm） with gradient elution of acetonitrile and water containing 0.1% trifluoroacetic acid at the flow rate of 1.0 mL·min-1.The detection wavelength was 214 nm.The BITC was detected by GC chromatography with SPB1701 capillary column（30 m×0.32 mm×0.25 μm） programmed temperature,the nitrogen as carrier gas.The FID detector temperature was 280 ℃,injector temperature was 250 ℃.Results：The BG and BITC had good linearity in the range of 0.91-36.4 μg（r=0.9992）,0.0174-0.556 μg（r=0.9992）,respectively.BG could be detected in Carica papaya organ except mature pulp.The highest content was 4.74 mg·g-1 in its seed.While BITC could be detected in flower and seed,higher content was 0.605 mg·g-1 in seed.Conclusion：This methods can be applied for the determination of the content of BG and BITC in Carica papaya.

关 键 词：番木瓜 异硫氰酸苄酯 苄基硫代葡萄糖苷 高效液相色谱法 气相色谱法 

分 类 号：R917[医药卫生—药物分析学]