鲁米诺-Ag(Ⅲ)化学发光新体系用于石杉碱甲的测定  

Determination of huperzine A by a new luminol-Ag(Ⅲ) chemiluminescence system

作　　者：李占华[1] 刘菲[2] 石红梅[2] 康维钧[2] 徐向东[2] 

机构地区：[1]邢台高等医学专科学校药理学教研室,邢台054000 [2]河北医科大学公共卫生学院,石家庄050017

出　　处：《药物分析杂志》2011年第4期690-693,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定石杉碱甲的流动注射化学发光法。方法:在碱性介质中,石杉碱甲对鲁米诺-Ag(Ⅲ)化学发光体系有显著的增敏作用,建立了化学发光检测石杉碱甲的新方法。结果:在优化的条件下,石杉碱甲浓度在1.0×10-8～5.0×10-7mol·L-1范围内与化学发光强度呈线性关系;对浓度为2.0×10-7 mol·L-1的石杉碱甲溶液11次平行测定的RSD为2.6%;检出限为8.0×10-9 mol·L-1。结论:该方法简单、快速、灵敏,适用于药物制剂中石杉碱甲的含量测定。Objective：To establish a novel chemiluminescence（CL） method for the determination of huperzine A.Method：Based on the fact that huperzine A could dramatically enhance the CL reaction of luminol with Ag（Ⅲ） complex in alkaline solution,a novel method for the determination of huperzine A was established.Results：Under optimum conditions,CL intensities were proportional to the concentration of huperzine A in the 1.0×10-8-5.0×10-7 mol·L-1 range.The detection limit was 8.0×10-9 mol·L-1,with a relative standard deviation（n=11） of 2.6% for 2.0 × 10-7 mol·L-1 huperzine A.Conclusion：This method is simple,fast,and sensitive for the determination of huperzine A in tablets.

关 键 词：化学发光 流动注射 石杉碱甲 Ag(Ⅲ) 

分 类 号：R917[医药卫生—药物分析学]