柽柳硫氧还蛋白过氧化物酶(ThPrx1)基因转入酵母的抗逆能力分析  

Stress Tolerance of Thioredoxin Peroxidase Gene from Tamarix hispida(ThPrx1) Inserted into Yeast

作　　者：贺琳[1] 蒋丽丽[1] 王玉成[1] 

机构地区：[1]林木遗传育种与生物技术教育部重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2011年第4期101-104,共4页Journal of Northeast Forestry University

基　　金：国家自然科学基金(30972387)资助

摘　　要：从柽柳(Tamarix hispida)中克隆到了一个硫氧还蛋白过氧化物酶(Peroxiredoxin,Prx)家族基因(ThPrx1)。为了研究ThPrx1的抗逆功能,将ThPrx1基因构建到酵母表达载体pYES2中,转化到酿酒酵母(Saccha-romyces cerevisiae)中,获得重组酵母,并用转空pYES2质粒的酵母作为对照。分别用NaCl、KCl、高温、低温、Cu-SO4、CdCl2、山梨醇、Na2CO3、甲基紫精胁迫条件处理转ThPrx1基因酵母(pYES2-ThPrx1)与对照(pYES2)酵母,来检测ThPrx1基因的抗逆能力。结果表明,ThPrx1基因具有抗NaCl、KCl、高温、低温、CuSO4、CdCl2、山梨醇、Na2CO3、甲基紫精胁迫的能力。研究结果提示该基因参与了柽柳的抗逆调控过程。Full-length cDNA of Thioredoxin Peroxidase Gene（Prx1） was cloned from Tamarix hispida.To investigate the stress tolerance of PRX1 from T.hispida（ThPrx1）,the recombinant plasmid designated as pYES2-ThPrx1 was constructed by inserting the ThPrx1 gene into the yeast expression vector pYES2.The plasmid pYES2-ThPrx1 was transformed into the yeast Saccharomyces cerevisiae,and the yeast transformed with empty pYES2 was used as the control.The recombinant yeast harboring ThPrx1 and the control yeast harboring empty pYES2 were treated with NaCl,KCl,high temperature,low temperature,CuSO4,CdCl2,sorbitol,Na2CO3 and methyl viologen,respectively.Results indicated that the ThPrx1 was tolerant to NaCl,KCl,high temperature,low temperature,CuSO4,CdCl2,sorbitol,Na2CO3 and methyl viologen.ThPrx1 might play a role in stress tolerance of T.hispida.

关 键 词：柽柳 硫氧还蛋白过氧化物酶 抗逆 酵母表达 

分 类 号：S722.36[农业科学—林木遗传育种]