调肝运脾汤治疗腹泻型肠易激综合征的实验研究  

Experimental Research on Modifying Liver and Spleen Decoction for Diarrhea-predominant IBD

作　　者：刘春[1] 叶柏[2] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210046 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《南京中医药大学学报》2011年第2期151-154,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省社会发展基金(BS2007094)

摘　　要：目的观察调肝运脾汤对腹泻型肠易激综合征(D-IBS)大鼠模型体质量、小肠推进率、部分脑肠肽含量的影响。方法采用番泻叶煎剂灌胃及夹尾刺激建立腹泻型IBS大鼠模型,实验设空白组、模型组、调肝运脾汤高、中、低剂量组、得舒特组,实验结束后测小肠推进率、结肠组织和血清5-羟色胺(5-HT)含量、结肠组织P物质(SP)、血管活性肽(VIP)含量。结果调肝运脾汤可明显改善由番泻叶引起的动物腹泻症状,使动物体质量增长,减缓小肠推进率;可明显降低血清和组织中5-HT含量,降低组织中SP、VIP含量,与模型组比较,差异显著(P<0.05,P<0.01);模型组大鼠血清5-HT含量及结肠5-HT、SP、VIP含量呈上升趋势,与空白组比较有显著差异(P<0.05,P<0.01)。结论调肝运脾汤可能通过降低血清5-HT含量及结肠组织5-HT、VIP、SP含量,从而改善胃肠动力,发挥治疗肠易激综合征的效应。OBJECTIVE To observe the effect of Modifying Liver and Spleen Decoction（MLSD） on the weight,gastric emptying rate,and the content of braingut petide of the model rats with diarrhea-predominant irritable bowel syndrome（D-IBS）.METHODS Model rats were under intragastric administration of senna leaf decoction and stimulation with forceps clip on tails.There were sham group,model group,MLSD groups of high,medium and low dose,and Deshute group.We examined gastric emptying rate,5-HT content in colon tissue and serum,SP in colon tissue as well as the content of VIP.RESULTS MLSD significantly improved diarrhea induced by senna leaf,which raised the weight of the model rats,decreased the gastric emptying rate,greatly reduced the content of 5-HT in serum and tissue as well as SP and VIP content.Compared with the model group,there was a clear difference（P0.05 or P0.01）.Besides,an increasing trend of 5-HT in serum and 5-HT,SP,and VIP in colon indicated great difference between the sham group（P0.05 or P0.01）.CONCLUSION MLSD may cure IBS by lowering 5-HT in serum and 5-HT,SP,and VIP in colon tissue to improve gastrointestinal motility.

关 键 词：调肝运脾汤 肠易激综合征 腹泻型IBS大鼠模型 小肠推进率 5-HT VIP SP 

分 类 号：R285.5[医药卫生—中药学]