EPO对新生大鼠缺氧缺血性脑损伤时脑组织Caspase-9和Caspase-3的影响  

Effect of Exogenous Erythropoietin on Caspase-9 and Caspase-3 Expression in Newborn Rats after Hypoxia-ischemia Brain Damage

作　　者：白丹[1] 阴怀清[2] 阴崇娟[2] 武师润[2] 

机构地区：[1]山西医科大学 [2]山西医科大学第一医院

出　　处：《中西医结合心脑血管病杂志》2011年第4期466-468,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：山西省太原市科技局科技兴市基金资助项目(No.0705026)

摘　　要：目的观察新生大鼠缺氧缺血性脑损伤(HIBD)时脑组织Caspase-9和Caspase-3的表达变化及促红细胞细胞生成素(EPO)对其表达的影响,从而探讨EPO发挥脑保护作用的可能机制。方法将新生7 d SD大鼠120只随机分成3组,假手术组、HIBD组r、hEPO治疗组,每组根据不同时间点又分为5个亚组:6 h组、12 h组、24 h组、48 h组、72 h组,每组8只,用免疫组化的方法观察各组脑组织Caspase-9和Caspase-3的表达。结果 Caspase-9的表达在缺氧缺血6 h即增强,12 h时逐渐升高,24 h^72 h维持在高峰水平(P<0.01);Caspase-3的表达也在缺氧缺血6 h即增强,12 h时逐渐升高,24 h^48 h达高峰,72 h稍有降低(P<0.01);rhEPO治疗组各时间点Caspase-9和Caspase-3的表达水平较HIBD组均明显降低(P<0.01)。结论 Caspase-9和Caspase-3参与了新生大鼠脑组织HIBD的发生发展过程,EPO可能通过降低新生大鼠缺氧缺血性脑损伤时脑组织Caspase-9和Caspase-3的表达发挥其脑保护作用。Objective To observe the expression of caspase-9 and caspase-3 in brain tissue of neonatal rats after hypoxia-ischemia brain damage（HIBD） and the effects of exogenous erythropoietin（EPO） on the expression of caspase-9 and caspase-3.Methods One hundred and twenty seven-day-old neonatal Sprague-Dawley rats were randomly divided into three groups：Sham-operated group,HIBD group and rhEPO treatment group.Each group was further divided into five sub-groups（n=8） based on different time points after HIBD（6 h,12 h,24 h,48 h,72 h）,respectively.Immunohistochemical technique was used to determine the expression of caspase-9 and caspase-3 in brain tissues.Results The expression of caspase-9 in HIBD group was increased at 6 h after HIBD,increased gradually at 12 h,and maintained the peak level 24 h to 72 h（P〈0.01）.The expression of caspase-3 in HIBD group was increased at 6 h after HIBD,increased gradually at 12 h,reached the peak at 24 h to 48 h,and decreased gradually at 72 h（P〈0.01）.The expression of caspase-9 and caspase-3 in rhEPO treatment group was significantly lower than that in HIBD group at each time points（P〈0.01）.Conclusion Caspase-9 and caspase-3 might participate in the occurrence and development process of HIBD in neonatal rats.EPO might reduce the expression of caspase-9 and caspase-3 in brain tissue after HIBD.EPO had some neuroprotective effects on the brain tissues in neonatal rats with HIBD.

关 键 词：促红细胞细胞生成素 缺氧缺血性脑损伤 CASPASE-9 CASPASE-3 

分 类 号：R722.1[医药卫生—儿科]