一种快速微量提取柑橘早期胚基因组DNA的方法  

A Rapid Method for the Isolation of Small Amount DNA from Citrus Early Embryo

作　　者：范达[1,2,3] 雷天刚[1,2,3] 王军政[1,2,3] 宋二玲[1,2,3] 彭爱红[1,2,3] 谭洪泉[1,2,3] 王金萍[1,2,3] 李政利[1,2,3] 李凤龙[1,2,3] 陈勇[1,2,3] 陈善春[1,2,3] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]西南大学柑橘研究所,重庆400712 [3]国家柑橘品种改良中心,重庆400712

出　　处：《安徽农业科学》2011年第9期5089-5090,共2页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划专项(2007BAD47B07-02);重庆市重大科技专项(CSTC;2007AB1040)

摘　　要：[目的]建立一种快速微量提取柑橘基因组DNA的方法。[方法]采用优化的CTAB微量提取法,以20.0、10.0、5.0、2.5 mg早期杂种胚为材料进行基因组DNA的提取,并对其DNA进行质量检测和SSR验证。[结果]该方法简单易行,所需材料少,提取的DNA纯度较高,OD260nm/OD280nm在1.800～2.000,可满足SSR等以PCR扩增为基础的试验需要。[结论]建立的方法可以用于快速微量提取柑橘基因组DNA。[Objective] The present study aimed to establish a rapid method for the isolation of small amount DNA from citrus.[Method] By using the improved CTBA method,the genomic DNA was extracted respectively from 20.0,10.0,5.0 and 2.5 mg hybrid embryos of citrus,and then the DNA quality was detected and followed by SSR verification.[Result] The method was very simple and rapid,which needed less materials.In addition,the isolated DNA showed good purity with the OD260/OD280 of 1.800-2.000,and could meet the requirement for PCR-based technology,such as SSR,etc..[Conclusion] The method could be used for rapid extraction of small amount of genomic DNA from citrus.

关 键 词：柑橘基因组 CTAB法 微量提取 PCR 

分 类 号：S183[农业科学—农业基础科学]