一氧化氮在有氧运动大鼠股四头肌过氧化应激及JNK表达中的作用  

Role of Nitric Oxide in Oxidative Stress and JNK Expression of Quadriceps Femoris of Rats in Aerobic Exercise

作　　者：秦春霞[1] 钱海[1] 车力龙[1] 秦从军[1] 陈谦[1] 肖德生[2] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013 [2]广州医学院公共卫生与全科医学学院,广东广州510182

出　　处：《沈阳体育学院学报》2011年第1期58-61,共4页Journal of Shenyang Sport University

基　　金：国家自然科学基金项目(30570894;30270639)

摘　　要：目的:探讨有氧运动后一氧化氮(NO)对股四头肌的过氧化应激及MAPK通路的调控作用。方法:大鼠分为静息组(SED)、运动组(EXE)、静息+NOS抑制组(SED-LNAME)、运动+NOS抑制组(EXE-LNAME)。运动组及运动+NOS抑制组每周连续游泳5d。共持续3个月。观察大鼠股四头肌MDA、SOD、TAOC及JNK水平的变化。结果:与SED组比较,EXE组大鼠股四头肌SOD、TAOC、p-JNK蛋白显著升高,在应用L-NAME干预后,运动+NOS抑制组MDA升高,SOD、TAOC、p-JNK显著下降,t-JNK无显著性变化。结论:运动训练诱导产生的NO可能介导JNK信号激酶诱导抗氧化酶产生,从而提高骨骼肌的抗氧化能力。Objective：To investigate the role of NO（nitric oxide） in oxidative stress and the MAPK pathways of quadriceps femoris in long-term aerobic exercise.Methods：Rats were divided into a sedentary group（SED）,an exercise group（EXE）,a sedentary plus L-NAME group（SED-LNAME） and an exercise plus L-NAME group（EXE-LNAME）.The rats in the EXE group and EXE plus LANME group swam for 5 days in a week,lasting for 3 months,and then the levels of MDA（malonaldehyde）,SOD（Superoxide Dismutase）,TAOC（total antioxidant capacity） and JNK（c-Jun N-terminal kinase） in the quadriceps femoris were analyzed.Results：Compared with the sedentary group,the contents of SOD,TAOC and p-JNK（phosphorylation-JNK） of the exercise group were significantly increased in quadriceps femoris.Treatment with L-NAME led to an increase in the MDA content,a significant decrease in the SOD,TAOC and p-JNK levels,without significant changes of the level of t-JNK in the EXE-LNAME group.Conclusion：During long-term aerobic exercise,NO may improve antioxidant capacity in skeletal muscle by increasing antioxidant enzymes activities,possibly through activating the JNK pathway.

关 键 词：运动 抗氧化 NO JNK 

分 类 号：G804.23[文化科学—运动人体科学]