抗堆型艾美球虫子孢子单克隆抗体杂交瘤细胞的复苏及其活性的初步鉴定  

Recovery and Initial Identification of the Hybridoma Cells Secreting McAbs Against Sporozoites of E.acervulina

作　　者：康桂英[1] 秦建华[2] 翟丽娟[1] 钟罡 赵静雯[1] 梅花[1] 

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028000 [2]河北农业大学动物科技学院,河北保定071000 [3]内蒙古家畜改良工作站,内蒙古呼和浩特010011

出　　处：《内蒙古民族大学学报（自然科学版）》2011年第1期52-54,共3页Journal of Inner Mongolia Minzu University：Natural Sciences

摘　　要：将堆型艾美耳球虫卵囊孢子化、破碎、脱去孢子囊、纯化子孢子并将其超声裂解,最终离心得到子孢子可溶性抗原,经紫外分光光度法测得抗原样品的浓度为1.58mg/mL.将保存的杂交瘤细胞解冻,培养并利用其制备抗体.用制备的抗原对此抗体进行了初步鉴定.E.acervulina oocysts were rejuvenated,purificated and sporulated,sporozoites from broken sporangium were purificated and sonicated,Soluble antigen of Plasmodium was obtained from Ultrasonic degradation products.concentration of antigen samples measured by UV spectrophotometry is 1.58mg/mL.The hybridoma cell was thawed,cultured,Antibodies were pr-epared using hybridoma cell and identified with soluble antigen.

关 键 词：堆型艾美耳球虫 杂交瘤细胞 子孢子 

分 类 号：Q78[生物学—分子生物学]