Inhibitory Effects of Natural Compound Alantolactone on Human Non-small Cell Lung Cancer A549 Cells  

作　　者：ZONG Min-ru ZHAO Ying-hao ZHANG Kun YANG Long-fei ZHENG Yong-chen HE Cheng-yan 

机构地区：[1]China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China [2]The Second Hospital of Jilin University, Changchun 130041, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第2期241-244,共4页高等学校化学研究（英文版）

基　　金：Supported by the Natural Science Foundation of Jilin Province; China(No.20090461)

摘　　要：Alantolactone is a natural compound identified from the roots of Inula helenium L. that has multiple bio-activities. We examined its inhibitory effects on human non-small cell lung cancer（NSCLC） A549 cells. The an-tiproliferative effect of alantolactone on A549 cells was investigated via MTT[3′-（4,5dimethylthiazol-2-yl）-2,5- diphenyl tetrazolium bromide] assay and its apoptosis-inducing effect was determined by Hoechst staining and flow cytometry. We found that alantolactone significantly inhibited the proliferation of A549 cells and induced morphological changes typical for apoptosis. Flow cytometry analysis indicates dose-dependent cell cycle retardation at G0/G1 and S stages. The results indicate that alantolactone could be an attractive small-molecular natural compound for further development as a therapeutic drug against NSCLC.Alantolactone is a natural compound identified from the roots of Inula helenium L. that has multiple bio-activities. We examined its inhibitory effects on human non-small cell lung cancer（NSCLC） A549 cells. The an-tiproliferative effect of alantolactone on A549 cells was investigated via MTT[3′-（4,5dimethylthiazol-2-yl）-2,5- diphenyl tetrazolium bromide] assay and its apoptosis-inducing effect was determined by Hoechst staining and flow cytometry. We found that alantolactone significantly inhibited the proliferation of A549 cells and induced morphological changes typical for apoptosis. Flow cytometry analysis indicates dose-dependent cell cycle retardation at G0/G1 and S stages. The results indicate that alantolactone could be an attractive small-molecular natural compound for further development as a therapeutic drug against NSCLC.

关 键 词：ALANTOLACTONE Non-small cell lung cancer（NSCLC） A549 PROLIFERATION APOPTOSIS 

分 类 号：S852.43[农业科学—基础兽医学] Q255[农业科学—兽医学]