Electrospun Poly(acrylonitrile-co-acrylic acid) Nanofibrous Membranes for Catalase Immobilization:Effect of Porphyrin Filling on the Enzyme Activity  

作　　者：KE Bei-bei WAN Ling-shu HUANG Xiao-jun XU Zhi-kang 

机构地区：[1]Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education,Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第2期339-344,共6页高等学校化学研究（英文版）

基　　金：Supported by the National High-Tech Research and Development Program of China(No.2007AA10Z301) ;the National Natural Science Foundation of China for Distinguished Young Scholars(No.50625309)

摘　　要：Porphyrin-filled nanofibrous membranes were facilely prepared by electrospinning of the mixtures of poly（acrylonitrile-co-acrylic acid）（PANCAA） and porphyrins. 5,10,15,20-Tetraphenylporphyrin（TPP） and its metal-loderivatives（ZnTPP and CuTPP） were studied as filling mediators for the immobilization of redox enzyme. Results indicate that the introduction of TPP, ZnTPP and CuTPP improves the retention activity of the immobilized catalase. Among these three porphyrins, the ZnTPP-filled PANCAA nanofibrous membrane exhibits an activity retention of 93%, which is an exciting improvement. This improvement is attributed to both the strong catalase-porphyrin affinity and the possible facilitated electron transfer induced by the porphyrin as evidenced by quartz crystal microbalance （QCM） and fluorescence spectroscopy studies.Porphyrin-filled nanofibrous membranes were facilely prepared by electrospinning of the mixtures of poly（acrylonitrile-co-acrylic acid）（PANCAA） and porphyrins. 5,10,15,20-Tetraphenylporphyrin（TPP） and its metal-loderivatives（ZnTPP and CuTPP） were studied as filling mediators for the immobilization of redox enzyme. Results indicate that the introduction of TPP, ZnTPP and CuTPP improves the retention activity of the immobilized catalase. Among these three porphyrins, the ZnTPP-filled PANCAA nanofibrous membrane exhibits an activity retention of 93%, which is an exciting improvement. This improvement is attributed to both the strong catalase-porphyrin affinity and the possible facilitated electron transfer induced by the porphyrin as evidenced by quartz crystal microbalance （QCM） and fluorescence spectroscopy studies.

关 键 词：Enzyme immobilization Nanofibrous membrane Electrospinning Quartz crystal microbalance（QCM） PORPHYRIN 

分 类 号：O626.13[理学—有机化学] TB3[理学—化学]