机械性创伤致急性肺损伤大鼠血清肿瘤坏死因子α的动态变化和机制  

The dynamic changes of serum tumor necrosis factor-α and the clinical significance in acute lung injury rats caused by mechanical trauma

作　　者：戴文娟[1] 贾国平[1] 刘卓拉[1] 高晓玲[1] 

机构地区：[1]山西医科大学第二医院呼吸内科,太原030001

出　　处：《中国医药》2011年第4期395-396,共2页China Medicine

基　　金：山西医科大学第二医院博士基金（20090407）

摘　　要：目的 探讨血清肿瘤坏死因子α（TNF-α）在机械性创伤致急性肺损伤大鼠中的动态变化和机制.方法 将雄性Wistar大鼠40只完全随机分为伪创伤组和创伤组、伪急性肺损伤组和急性肺损伤组,每组10只,利用Noble-Collip创伤仪制备机械性创伤模型,腹腔注射TNF-α制作急性肺损伤模型.全部大鼠均于造模后6 h腹主动脉采血处死,采用ELISA法测定血清TNF-α含量,肺组织行常规组织病理学检查.结果 创伤组大鼠血清TNF-α含量明显高于伪创伤组[（335±28）mg/L比（177±10）ng/L],差异有统计学意义（P＜0.01）.病理结果 显示：创伤组和急性肺损伤组肺组织炎症非常明显,而伪创伤组和伪急性肺损伤组基本正常.结论 机械性创伤大鼠血清中TNF-α含量的升高可能与机械性创伤后急性肺损伤有关.动态观察机械性创伤后血清中TNF-α的变化,有助于病情的评估及预后判断,具有重要的临床意义.Objective To determine the dynamic changes and mechanism of tumor necrosis factor-α（TNF-α） in serum in acute lung injury（ALI） induced by mechanical trauma in mechanical trauma rat model. Methods Totally 40 male Wistar rats were randomly divided into sham group and trauma group, sham-acute lung injury group and acute lung injury group. Noble-Collip drum was used to establish mechanical trauma rat model. Intraperitoneal injection of TNF-α established acute lung injury model. All rats after modeling of abdominal aortic blood sampling time points were killed and the serum levels of TNF-α were asasyed by ELISA. Results Serum TNF-α levels （334. 78 ±± 28） ng/L in the trauma group were significantly higher than those in the sham group（ 177 ±10 ） ng/L （P 〈0. 01 ）. The pathological results showed that both in trauma group and acute lung injury group lung infection were very obvious, while it was basically normal in both sham group and sham-acute lung injury group. Conclusions Serum TNF-αt levels of mechanical trauma may be associated with acute lung injury after mechanical trauma. Therefore, dynamic observation of the change of serum TNF-α after mechanical trauma will help the assessment and prognosis of the disease, it has important clinical significance.

关 键 词：急性肺损伤 机械性创伤 肿瘤坏死因子Α 

分 类 号：R563.9[医药卫生—呼吸系统]