100株住院儿童大肠埃希菌Ⅰ类整合子研究  

Studies on class Ⅰ integron carriage among 100 Escherichia coli isolates from clinical children

作　　者：高迎春 魏秀青 魏秀丽 庞晓飞[3] 都业良[4] 肖永霞[4] 刘兴莉[5] 王燕 张传津 苏梅 

机构地区：[1]山东省兽药质量检验所,山东济南250022 [2]山东省茌平县医院,山东茌平252100 [3]山东省立医院西院,山东济南250022 [4]青岛农业大学,山东青岛266109 [5]山东大学齐鲁儿童医院,山东济南250022

出　　处：《中国药物应用与监测》2011年第2期75-78,共4页Chinese Journal of Drug Application and Monitoring

基　　金：十一五"国家科技支撑计划"重大项目"抗耐药菌中兽药的研制与开发(2008BADB4B04)

摘　　要：目的:了解Ⅰ类整合子在住院儿童分离大肠埃希菌中分布流行情况。方法:根据NCCLS推荐的纸片扩散法进行药敏试验;PCR扩增临床大肠埃希菌Ⅰ类整合子整合酶基因和可变区基因盒。结果:患者整合酶检出率68%,Ⅰ类整合子基因盒检出率为65%。Ⅰ类整合子的大小约为772 bp～2360 bp,100株细菌各含1～2个Ⅰ类整合子,整合子中最常见的基因盒排列为dfrA17+aadA5和dfrA12+aadA2。结论:Ⅰ类整合子在多重耐药大肠埃希菌中广泛流行,是介导细菌多重耐药性的重要分子机制,应加强基因水平耐药监测。Objective：To investigate the occurrence and distribution of class I integron among 100 Escherichia coli isolates from clinical children.Methods：Antibacterial susceptibility testing was conducted by agar dilution test according to NCCLS.ClassⅠintegrase gene PCR was performed to detect the presence of integron.Results：The positive rate of class Ⅰ integrase and integron were 68% and 65% respectively.The size of class Ⅰ integrons ranged from 772 bp to 2360 bp.Each of the 100 isolates had 1 or 2 class Ⅰ integrons.The main order of inserted gene cassettes was dfrA17 ＋ aadA5 and dfrA12 ＋ aadA2.Conclusion：Class Ⅰ integrons are widespread in isolates of multidrug resistant E.coli.Integrons are important molecular mechanism in the development of multidrug resistance.The results stressed the need for surveillance of bacteria in genetic levels.

关 键 词：耐药性 整合子 基因盒 大肠埃希菌 

分 类 号：R378[医药卫生—病原生物学]