Wort mannin对细胞的辐射增敏作用  

Cellular radiosensitization by wortmannin\

作　　者：李雨[1] 鲁颖 郑秀龙[1] 罗成基[3] 蔡建明[1] 孟祥顺[1] 赵芳[1] 高建国[1] 赵岚[1] 

机构地区：[1]第二军医大学放射医学教研室,上海200433 [2]开封高等医学专科学校 [3]第三军医大学防原医学教研室

出　　处：《中华放射医学与防护杂志》1999年第5期330-333,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的　研究磷脂酰肌醇３激酶的特异性抑制剂Ｗｏｒｔｍａｎｎｉｎ（ ＷＴ） 对细胞的放射增敏效应，以探索放射增敏信号传导途径。方法　采用体外细胞克隆培养法观察不同浓度ＷＴ 在不同时间对３ 种细胞的放射增敏效应。结果　５０ μｍｏｌ／Ｌ以上浓度的ＷＴ 在照射后６ 小时内对ＬＰ３ 细胞呈较明显的增敏效应，剂量存活曲线Ｄ０ 增敏比在２ 左右；５０ μｍｏｌ／Ｌ ＷＴ 对ＨｅＬａ 细胞和ＳＰ／２０细胞均呈明确增敏效应。结论　ＷＴ是一种新型放射增敏剂。Objective\ To examine the radiosensitization possibility of wortmannin(WT) on some cell lines. Methods Cells were prepared with WT or DMSO(solvent) for 1h,and irradiated at the indicated dose.At different times after irradiation,the cells were washed to remove the WT and allowed to grow for 7 days,and colonies of more than 50 cells were examined under low power microscope. Results Marked radiosensitizing effect of WT in concentration over 50 μmol/L on LP cells within 6 h after radiation exposure was displayed,the D\-0 radiosensitization ratio in the dose survival curve being around 2.WT in concentration 50 μmol/L had definite radiosensitizing effect on HeLa and SP/20 cells. Conclusion WT is a noveldiosensitizer.\;

关 键 词：WORTMANNIN 放射增敏 细胞 PI-3K AT 

分 类 号：R811.5[医药卫生—放射医学]