石榴皮多酚凝胶的制备及质量控制  

Preparation and Quality Control of Pomegranate Polyphenols Gel

作　　者：严欢[1,2] 顾政一 陈孝娟[1] 刘宣麟[1] 马建红[1] 庞市宾[1] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]新疆维吾尔自治区药物研究所,乌鲁木齐830004

出　　处：《中国实验方剂学杂志》2011年第8期4-6,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(C191002)

摘　　要：目的:制备石榴皮多酚凝胶,建立其质量控制方法并考察其经皮渗透性。方法:以卡波姆-940为基质,甘油为润湿剂,氮酮为透皮吸收促进剂,山梨酸钾为防腐剂,三乙醇胺为中和剂,制备凝胶剂;采用紫外分光光度法测定石榴皮多酚凝胶中总多酚含量,并做小鼠离体皮肤经皮渗透试验。结果:凝胶制备工艺可行;没食子酸在0.024 5～0.245 0 g·L-1线性关系良好(r=0.999 6);平均回收率99.56%,RSD 0.58%。没食子酸24 h皮肤透过率为36.95%。结论:石榴皮多酚凝胶的制备方法简单,质量控制方法可靠,且有良好的渗透性。Objective：To prepare pomegranate polyphenols gel and control the quality and to observe penetrability of gallic acid from the gel in vitro.Method：Carbopol-940 was used as the gel matrix,glycerin as wetting agent,potassium sorbate as a preservative and triethanolamine as neutralizer.UV-spectrometry method was used to determine total polyphenol content of the gel.The skin of rat was put between the supplying container and receiving container,and the experimentation of penetrating was carried out.Result：The preparing technology of gels was feasible.Gallic acid can be completely separated within a linear range of 0.024 5-0.245 0 g.L-1（r=0.999 6）.The average recovery was 99.56%,RSD 0.58%.The rate of penetrability in Gallic acid is 36.95% during 24 hours.Conclusion：The preparation technique is simple and feasible for the quality control.It shows excellent transdermal effects.

关 键 词：石榴皮多酚 凝胶 卡波姆940 离体皮肤 渗透性 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]