不同治法方药对激素性股骨头坏死鸡股骨头OPG,RANKL mRNA表达的影响  

Effect of Different Treating Principles and Formulas on Expression of Osteoprotegerin and Receptor Activator of Nuclear Factor-κB Ligand mRNA in Steroid-induced Osteonecrosis of Femoral Head in Chichen

作　　者：万蓉[1] 李莉[2] 孔祥英[1] 肖春生[2] 姜宜妮[1] 林娜[1] 孔焕宇[2] 陈卫衡[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院望京医院,北京100102

出　　处：《中国实验方剂学杂志》2011年第8期149-153,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(30472131)

摘　　要：目的:观察比较健脾化痰、活血通络(简称健脾)与补肾壮骨、活血通络(简称补肾)2种治法对激素性股骨头坏死鸡股骨头骨保护素(osteoprotegerin,OPG)、NF-κB受体活化因子配基(receptor activator of nuclear factor-κB ligand,RANKL)mRNA表达的影响,探讨2者防治股骨头坏死的分子机制。方法:64只来杭鸡,随机分为对照、模型、健脾和补肾组,除对照组外均胸肌注射甲基泼尼松琥珀酸钠(5.2 mg·kg-1),健脾及补肾组在造模同时ig给予相应中药(6,9 g·kg-1.d-1),连续16周。给药8周和16周后,分批处死动物,取双侧股骨头,原位杂交法检测股骨头内OPG,RANKL mRNA的表达情况,并计算其比值。结果:与正常组相比,模型组8周和16周时股骨头内OPG阳性细胞数显著减少,分别为(778±189),(696±108)个,RANKL分别为(717±164),(519±162)个,明显增多(P<0.01);与模型组相比,健脾方在8周就可以明显上调OPG阳性细胞数达(1 049±211)个,同时也降低RANKL阳性细胞数至(454±166)个(P<0.05或P<0.01),升高OPG/RANKL比值(P<0.05),而补肾组此时作用不明显,16周时作用比健脾组强(P<0.05)。结论:健脾化痰、活血通络和补肾壮骨、活血通络2种不同治法方药均可调节股骨头内OPG和RANKL的基因表达,这可能是其有效抑制破骨细胞分化、防治激素性股骨头坏死的作用机制之一;健脾化痰、活血通络方药起效时间相对较早。Objective：To investigate the effect of two treating principles and formulas,named ‘invigorating spleen to remove phlem and promoting blood circulation to remove meridian obstruction’（Jianpi） and ‘invigoratethe kidney and promoting blood circulation to remove meridian obstruction ’（Bushen）,on the expression of osteoprotegerin（OPG） and receptor activator of nuclear factor-κB ligand（RANKL） mRNA in steroid-induced osteonecrosis of the femoral head（SONFH）,as well as to explore and compare their mechanism of prevention and treatment for SONFH.Method：Sixty-four chickens were randomly divided into control group,model group,Jianpi group and Bushen group.Except the control group,the animal model of SONFH was established with injection of methylprednisolone in chest muscle of chickens.At 8th and 16th weeks,both sides of femoral heads were taken off to observe the expression of OPG and RANKL mRNA with in situ hybridization staining.Result：Compared with the control group,the expression of OPG mRNA was decreased and RANKL mRNA increased significantly in model group（P 〈 0.01）.Compared with model group,both in Jianpi and Bushen group,the expression of OPG mRNA was increased and RANKL mRNA decreased significantly,meanwhile the ratio of OPG to RANKL was increased（P 〈 0.01 or P 〈 0.001）.In Bushen group,the change did not obvious at the 8th weeks,however,the changes were significant compared with that in Jianpi group at the 16th weeks.Conclusion：Both Jianpi and Bushen formula can regulate the expression of OPG and RANKL mRNA to inhibit bone destruction,thereby exert preventive and therapeutic effect for SONFH.Jianpi take action earlier than Bushen formula.

关 键 词：股骨头坏死 骨保护素 NF-KB受体活化因子配基 

分 类 号：R285.5[医药卫生—中药学]