复方青龙衣胶囊对胃癌细胞SGC-7901基因芯片表达的影响  

Changes in Gene Expression Profiles of Gastric Carcinoma after Treatment of Compound Qinglongyi Capsule

作　　者：许庆瑞[1] 张树明[1] 张俊威[1] 徐琛[2] 王伟明[1] 

机构地区：[1]黑龙江省中医研究院,哈尔滨150036 [2]南京大学生命科学院,南京210093

出　　处：《中国实验方剂学杂志》2011年第8期180-183,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：“重大新药创制”国家科技重大专项课题(2009ZX09102-138);黑龙江省发改委支持重大前期项目;黑龙江省青年科学基金项目(QC2010043)

摘　　要：目的:研究复方青龙衣胶囊体外对人胃癌细胞SGC-7901基因表达谱的影响,初步探讨其对胃癌细胞抑制作用的机制。方法:设定5个不同药物浓度梯度(0.8×10-3,1×10-3,1.2×10-3,1.4×10-3,1.6×10-3 g·L-1)预处理24 h,采用MTT法测定对人胃癌细胞SGC-7901的抑制作用;以药物半数抑制浓度预处理24 h,全基因芯片技术研究对胃癌基因表达谱的影响,并采用基因集合聚类分析法分析结果。结果:以不同浓度的复方青龙衣处理胃癌细胞SGC-7901 24 h,结果显示该药对肿瘤细胞的生长呈现剂量依赖性的抑制作用,半数抑制浓度为1.27×10-3g·L-1;芯片结果显示:胃癌肿瘤细胞SGC-7901经药物处理后总共筛选出78个差异表达基因,表达上调的基因有23个,表达下调的基因有55个。结论:复方青龙衣胶囊对人胃癌细胞SGC-7901具有显著的抑制作用,并可能通过影响细胞周期基因的表达,使肿瘤细胞停滞于G1/S期发挥抗肿瘤作用。Objective：To study the changes in gene expression profiles of gastric carcinoma after treatment of Compound Qinglongyi Capsule and the potential mechanism of it.Method：MTT assay was used to observe the effect of Compound Qinglongyi Capsule at doses of 0.8 × 10-3,1 × 10-3,1.2 × 10-3,1.4 × 10-3,1.6 × 10-3g.L-1 accordingly on proliferation of SGC-7901 cells.The gene expression spectra were tested by gene chip technique,and the databases were analyzed by gene set analysis.Result：MTT results showed that after treated with different doses for 24 h,Compound Qinglongyi Capsule could obviously inhibit the proliferation of SGC-7901 cells in a dose-dependent manner.Compared with the untreated group,expressions of 78 genes were changed,among them 23 gene expression up-regulated,and 55 gene expression down-regulated.Conclusion：Cell proliferation in the carcinoma cell line SGC-7901 can be inhibited effectively by Compound Qinglongyi Capsule.

关 键 词：胃癌 基因芯片 复方青龙衣胶囊 

分 类 号：R285.5[医药卫生—中药学]