芪附汤对阿霉素心脏毒性损伤的保护作用及其抗氧化机制  

Protective Effect and Antioxidant Mechanism of Qifu Decoction on Adriamycin-induced Cardiac Injury

作　　者：于彩娜[1] 范颖[1] 林庶茹[2] 

机构地区：[1]辽宁中医药大学方剂学科,沈阳110032 [2]辽宁中医药大学中医分子生物实验室,沈阳110032

出　　处：《中国实验方剂学杂志》2011年第8期193-196,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅高等学校创新团队项目(2007T117)

摘　　要：目的:观察芪附汤对阿霉素心脏毒性损伤模型大鼠心脏功能的影响,并初步探讨其作用机制。方法:采用心电图仪测定心脏功能的相关参数,采用化学比色法检测心肌细胞超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性及脂质过氧化产物丙二醛(malondialdehyde,MDA)含量。结果:与正常组相比较,模型组大鼠心率显著减慢,QRS波群电压明显降低,Q-T间期明显延长,均有统计学意义(P<0.05),个别出现心律失常;与模型组相比较,芪附汤组、中药对照组心率增加,QRS波群电压和明显增高,Q-T间期显著缩短,均有统计学意义(P<0.05)。与正常组相比较,模型组、芪附汤组、中药对照组心肌细胞SOD和GSH-Px活性减弱,MDA含量上升,均有统计学意义(P<0.05);与模型组相比较,芪附汤组、中药对照组心肌细胞SOD和GSH-Px活性增强,MDA含量下降,均有统计学意义(P<0.05)。结论:芪附汤对阿霉素诱导的大鼠心脏毒性损伤具有保护作用,作用机制与其抗氧化应激有关。Objective：To investigate the effect of Qifu Decoction on heart function of adriamycin-induced cardiac injury in a rat model,and to discuss the mechanism.Method：Heart function was measured by ECG instrument,the activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） were detected,and the content of lipid peroxidation product malondialdehyde（MDA） were checked by chemical colorimetric method.Result：Compared with the normal group,the heart rate of the rats in the model group was slowed down,the sum of QRS voltage decreased,Q-T interval extended,all with statistically significance（P 〈 0.05）.Arrhythmia appeared in a few individuals.Compared with the model group,the heart rate of the rats in Qifu Decoction group and Chinese herb control group was speeded up,the sum of QRS voltage increased,Q-T interval shortened,all with statistically significance（P 〈 0.05）.Compared with the normal group,the activities of SOD and GSH-Px were weakened,and the content of MDA increased in the model group,Qifu Decoction group and Chinese herb control group,all with statistically significance（P 〈 0.05）.Compared with the model group,the activities of SOD and GSH-Px were strengthened,and the content of MDA decreased in Qifu Decoction group and Chinese herb control group,all with statistically significance（P 〈 0.05）.Conclusion：Qifu Decoction can protect the heart from adriamycin in the rat model.Its mechanism may be related with antioxidant stress.

关 键 词：芪附汤 阿霉素 心脏毒性 氧化应激 

分 类 号：R285.5[医药卫生—中药学]