不同产地人参水煎剂对免疫抑制小鼠细胞免疫功能的影响  

Influences of Decoctions of Different places Ginseng on Cellular Immune Function in Immunosuppressed Mice

作　　者：雷萍[1] 关洪全[1] 王昊[1] 韩晓伟[1] 侯殿东[1] 陈殿学[1] 

机构地区：[1]辽宁中医药大学免疫病原生物教研室,沈阳110032

出　　处：《中国实验方剂学杂志》2011年第8期218-220,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅课题(2008456)

摘　　要：目的:比较辽宁、吉林、国外朝鲜不同产地人参对免疫抑制小鼠细胞免疫功能的影响。方法:皮下注射氢化可的松(70 mg·kg-1)制备免疫抑制动物模型,之后分别给予不同产地人参水煎剂(5 g·kg-1)灌胃,流式细胞术检测脾细胞悬液CD3,CD4,CD8;ELISA法检测血清IFN-γ含量;SRBC诱导小鼠迟发型超敏反应(DTH)。结果:经不同产地人参水煎剂治疗后,小鼠脾细胞悬液CD3,CD4,CD8均有升高。其中朝鲜人参能使免疫抑制小鼠CD4和CD4/CD8比值明显升高(P<0.05),辽宁人参与朝鲜人参和吉林人参相比并没有显著性差异;不同产地人参灌胃后DTH和IFN-γ也均显著升高(P<0.05),辽宁人参与朝鲜人参和吉林人参相比也并没有显著性差异。结论:辽宁人参水煎剂能提高免疫抑制小鼠的细胞免疫功能,与吉林人参和朝鲜人参相比并无明显差异。Objective：To compare the cellular immune function of Liaoning,Jilin and Korean ginseng in immunosuppressed mice.Method：Immunosuppressed model of mouse was induced by subcutaneous injection of hydrocortisone.Three decoctions of ginseng originated from Liaoning,Jilin and Korea were ig given to explore CD3,CD4,CD8 in spleen cell suspension by flow cytometry.Serum IFN-γ level was determined by ELISA assay;and SRBC was used to induce delayed-type hypersensitivity（DTH）.Result：The CD3,CD4 and CD8 of spleen cell suspension were increased after each ginseng decoction treatment,Korean ginseng could increase the CD4 and CD4/ CD8 ratio for the immunosuppressed mice significantly（P 〈 0.05）.Liaoning ginseng had no significant difference compared with that of Jilin ginseng or Korean ginseng.The levels of DTH and IFN-γwere also raised significantly after each decoction treatment（P 〈 0.05）,and Liaoning ginseng had no significant difference compared with that of Jilin ginseng or Korean ginseng.Conclusion：Liaoning ginseng decoction can improve cellular immune function on immunosuppressed mice,and Liaoning ginseng had no significant difference compared with Jilin and Korean ginseng.

关 键 词：人参水煎剂 免疫抑制 T细胞亚群 Γ-干扰素 迟发型超敏反应(DTH) 

分 类 号：R285.5[医药卫生—中药学]