人参养荣汤的急性毒性及抗疲乏抗应激的研究  

Acute Toxicity and Anti-stress Effect of Ginseng Tonic Decoction in Mice

作　　者：陈衍智[1] 林飞[2] 李萍萍[1] 

机构地区：[1]北京大学临床肿瘤学院北京肿瘤医院暨北京市肿瘤防治研究所中西医结合科恶性肿瘤发病机制及转化研究教育部重点实验室,北京100142 [2]中国药品生物制品检定所,北京100050

出　　处：《中国实验方剂学杂志》2011年第8期225-229,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市中医局科技项目(2004京中重IV15)

摘　　要：目的:观察人参养荣汤的急性毒性以及抗疲劳、抗应激作用。方法:选择SPF级KM种小鼠为受试对象,采用急性经口毒性试验测定最大耐受量,采用抗疲劳、耐缺氧以及耐高低温试验,进行抗应激作用的评价。结果:人参养荣汤的最大耐受量为>160 g生药/kg,相当于人临床每天口服用量的76.2倍。与空白对照组比较,人参养荣汤的高、中剂量组(按生药量计为42.0,21.0 g·kg-1)和十一味参芪片组(1 g·kg-1)可延长小鼠不间断走动疲劳过度从转棒上跌落的时间;人参养荣汤的高剂量组可延长小鼠断头后喘息时间和小鼠常压下的窒息时间;人参养荣汤的高、中剂量组和十一味参芪片组具有延长小鼠45℃高温下死亡的时间和小鼠-5℃低温下死亡时间,具有统计学意义(P<0.05或P<0.01)。结论:人参养荣汤的最大耐受量按生药量计>160 g·kg-1,相当于人临床每天口服用量的76.2倍。人参养荣汤可以提高小鼠的抗疲劳、耐缺氧和耐高低温能力,具有抗疲劳和抗应激作用。Objective：To oberseve the acute toxicity,anti-fatigue effect and anti-stress effect of Ginseng Tonic Decoction in mice.Method：Mice of KM species were used to test the maximum tolerance dose（MTD） of Ginseng Tonic Decoction.The mouse anti-fatigue test,the mouse acute cerebral ischemia anti-hypoxia test,the mouse anti-hypoxia test and the mouse resistant test to high and low-temperature were used to evaluate anti-stress effect of Ginseng Tonic Decoction at doses of 42.0,21.0 and 10.5 g.kg-1.d-1.Result：the oral MTD of Ginseng Tonic Decoction in mice was 〉 160 g.kg-1.d-1,equal to 76.2 times of human adult’s clinical dose per day.Compared with the control group,mice in Ginseng Tonic Decoction in high dosage group,moderate dosage group and positive control group could prolong the falling time that mice drop from rotating bar because of fatigue;Ginseng Tonic Decoction in high dosage group could prolong the asthmatic time after decapitation and the death time under ambient pressure in mice;Decoction in high dosage group,moderate dosage group and positive control group could prolong the death time under high temperature of 45 ℃ and under low temperature of-5 ℃,there were significant differences（P 〈 0.05 or P 〈 0.01）.Conclusion：Tonic Decoction can improve the ability of anti-fatigue,anti-hypoxia and resistance to high and low-temperature.So,the decoction shows anti-stress effects without detectable acute toxicity.

关 键 词：人参养荣汤 小鼠 急性毒性试验 抗疲劳作用 抗应激作用 

分 类 号：R285.5[医药卫生—中药学]