人工冬虫夏草不同部位核苷类成分分析  

Analysis of nucleoside constituents in different parts of the artificial Cordyceps sinensis

作　　者：陆艳艳[1] 邱细敏[1] 蒋诚[1] 刘胜姿[1] 郭思维[1] 陈文[2] 

机构地区：[1]湖南师范大学医学院药学系,长沙410006 [2]长沙医学院,长沙410219

出　　处：《食品科技》2011年第4期250-252,256,共4页Food Science and Technology

基　　金：国家大学生创新性实验项目(043-0094);湖南省教育厅重点项目(10A071)

摘　　要：目的:分析测定人工冬虫夏草不同部位核苷类含量,为充分利用人工冬虫夏草资源提供参考。方法:水提醇沉,RP-HPLC分离,紫外检测器检测虫草素、腺苷和腺嘌呤含量。结果:人工虫草中子实体、菌丝体和固体培养残基中腺嘌呤含量(μg/g)分别为193.92、142.66、71.25;腺苷分别1302.52、635.02、171.73;虫草素分别为1356.92、1101.04、350.96。结论:人工虫草中不但子实体含有腺嘌呤、腺苷和虫草素,菌丝体及固体培养残基也含有这3种主要核苷类物质,具有进一步开发利用价值。To determine nucleoside constituents in different parts of the artificial Cordyceps sinensis,to provide a reference for taking full advantage of artificial Cordyceps sinensis resources.Methods：Water extraction and alcohol precipitation,RP-HPLC Separation,quantitative analysis of cordycepin,adenosine and adenine using UV-Vis etector.Results：The content of adenine（μg/g） in the fruiting bodies,the mycelia of artificial Cordyceps and the solid culture Residues was 193.916,142.658,71.25,that of adenosine 1302.52,308.17,71.51,and that of cordycepin 1356.92,1101.04,350.96.Conclusion：Not only the fruiting bodies,but also the Mycelia of the artificial Cordyceps inensis and the solid culture residues contain adenine,adenosine and cordycepin,which is worthy to explore further for its useful value.

关 键 词：人工冬虫夏草 腺苷 腺嘌呤 虫草素 高效液相色谱法 含量测定 

分 类 号：TS567.35[轻工技术与工程—皮革化学与工程]