矿区分离丛枝菌根真菌对万寿菊吸Cd潜力影响  

Effects of arbuscular mycorrhizal fungi isolated from mining area on the enhancement of Cd uptake in marigold plants

作　　者：刘灵芝[1,2] 李培军[2] 巩宗强[2] 张玉龙[1] 

机构地区：[1]沈阳农业大学土地与环境学院,辽宁沈阳110866 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《微生物学通报》2011年第4期575-582,共8页Microbiology China

基　　金：国家自然科学基金重点项目(No.40930739);中国科学院知识创新工程重要方向项目(No.KZCX2-YW-446);中国科学院知识创新工程重大项目(No.kzcx1-yw-06-03)

摘　　要：盆栽试验研究土壤不同施Cd水平(0、5、20、50μg/g)下,接种矿区污染土壤中丛枝菌根真菌对万寿菊根系侵染率、植株生物量及Cd吸收与分配的影响。结果表明:接种丛枝菌根真菌显著提高Cd胁迫下万寿菊的根系侵染率和植株生物量;随着施Cd水平提高,各处理植株Cd浓度显著增加。各施Cd水平下万寿菊地上部Cd吸收量远远高于根系Cd吸收量,在土壤施Cd量达到50μg/g时,接种处理地上部Cd吸收量是根系的3.48倍,对照处理地上部Cd吸收量是根系的1.67倍;同一施Cd水平下接种处理植株Cd吸收量要显著高于对照。总体上,试验条件下污染土壤中分离的丛枝菌根真菌促进了万寿菊对土壤中Cd的吸收,并在一定程度上增加Cd向地上部分的运转,表现出植物提取的应用潜力。In a pot experiment,effects were investigated of inoculation with arbuscular mycorrhizal fungi isolated from soil of a mining area on root colonization and biomass,Cd uptake and translocation of Tagetes erecta L.under different Cd addition levels（0,5,20,50 μg/g）.Results indicated that my-corrhizal inoculation significanty increased root colonization and biomass of marigold plants.Plant Cd concentrations markedly increased with increasing Cd addition levels.For all treatments,shoot Cd contents were significantly higher than root Cd contents,while under each Cd addition level shoot Cd contents of inoculated plants were significantly higher than those of uninoculated controls.Especially,under Cd addition level of 50 μg/g,shoot to root ratio of Cd content in inoculated plants was 3.48,in contrast to 1.67 for uninoculated plants.In general,inoculation with arbuscular mycorrhizal fungi from contaminated soil can increase Cd uptake in plants and enhance Cd translocation from roots to shoots,showing promises in phytoextraction.

关 键 词：丛枝菌根真菌(AMF) 万寿菊 生物量 CD胁迫 

分 类 号：S681.9[农业科学—观赏园艺] X173[农业科学—园艺学]