纸芯片制作及其在化学发光法检测葡萄糖和尿酸中的应用  

The Fabrication and Its Analytical Applications of Paper-Based Microfluidics for The Detecting of Glucose and Uric Acid with Chemiluminescent Image

作　　者：王方方[1] 陈锦[1] 何治柯[1] 

机构地区：[1]武汉大学化学与分子科学学院,生物医学分析化学教育部重点实验室,湖北武汉430072

出　　处：《分析科学学报》2011年第2期137-141,共5页Journal of Analytical Science

基　　金：国家973计划(No.2007CB714507)

摘　　要：纸芯片是一类新型的个性化诊断器件,可快速、廉价、简便地进行多组分分析。采用光刻法制备了一维和二维纸芯片(μPADs),并对不同通道芯片检测所需的样品体积进行理论估算。同时利用葡萄糖氧化酶-葡萄糖反应产生过氧化氢诱导鲁米诺化学发光,在纸芯片上检测葡萄糖,线性范围为2.0×10-4～9.0×10-4 mol/L,检出限为5.0×10-5 mol/L,比纸芯片上电化学检测葡萄糖(5.0×10-4 mol/L)低一个数量级。利用同样的原理进行了尿酸检测,线性范围为1.0×10-4～1.2×10-3 mol/L,检出限为6.0×10-5 mol/L,检测0.5mmol/L葡萄糖和0.5mmol/L尿酸的相对标准偏差分别为2.68%(n=11)和4.59%(n=11)。将一维、二维芯片组装成三维芯片,验证了纸芯片上化学发光法同时测定葡萄糖和尿酸的可能性。Microfluidic paper-based analytical devices（μPADs）,a new class of point-of-care diagnostic devices,provide an exciting platform for the rapid,low-cost,and technically simple multiplexed bioassays.In this work,photolithography was used to pattern the paper.An equation was obtained to describe the relationship between the volume of samples loaded and the volume of the channels.The analytical application was demonstrated by the detection of glucose and uric acid based on the enzyme induced chemiluminescence of luminol system.Under the optimized conditions,the chemiluminescence（CL） intensity is linear with glucose concentration over the range of 2.0×10-4～9.0×10-4 mol/L with a limit of detection（LOD） of 5.0×10-5 mol/L.The LOD for glucose was found to be substantially lower than that of the electrochemical method（5.0×10-4 mol/L） on μPADs.In the same principle,the CL intensity is linear with uric acid concentration over the range of 1.0×10-4～1.2×10-3 mol/L with a LOD of 6.0×10-5 mol/L.The LOD for uric acid was found to be two orders of magnitude lower than that of the electrochemical method（1.38×10-3 mol/L） on μPADs.1D and 2D μPADs have also assembled into 3D μPADs to detect glucose and uric acid simultaneously.

关 键 词：纸芯片 化学发光 葡萄糖 尿酸 

分 类 号：O657.39[理学—分析化学]