阿莫西林与玫瑰精B的共振瑞利散射光谱及应用  

Resonance Rayleigh Scattering Spectra for Studying the Interaction of Amoxicillin with Rhodamine B

作　　者：庞向东[1] 郑晓锋[1] 邓钦心[1] 江虹[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《分析科学学报》2011年第2期215-218,共4页Journal of Analytical Science

基　　金：长江师范学院科研项目(No.2008xky39)

摘　　要：利用共振瑞利散射法测定阿莫西林的含量。在pH=9.5～9.9的Tris-HCl缓冲溶液中,阿莫西林和玫瑰精B作用后,共振瑞利散射显著增强,在306～378nm波长范围内呈现高的散射强度,并以340和372nm波长处的△IRRS最强。阿莫西林的质量浓度在0～0.4μg/mL范围内与△IRRS呈正比,据此建立了测定阿莫西林的共振瑞利散射法,检出限(3Sb/S)为0.045μg/mL。该法简便、快速、灵敏,用于痕量阿莫西林的测定,结果满意。The determination of amoxicillin was performed by resonance Rayleigh scattering（RRS） method.In pH=9.5～9.9 Tris-HCl buffer solution,the RRS intensity of rhodamine B in the range of 306～378 nm was greatly enhanced when amoxicillin was added,and the strongest △IRRS was located at 340 and 372 nm.The intensity of RRS was in proportion to the concentration of amoxicillin in the range of 0～0.4 μg/mL.A sensitive RRS method for the determination of amoxicillin has been developed with a detection limit（3Sb/S） of 0.045 μg/mL.The method showed advantages of convenience,rapidity,high sensitivity and was applied to the determination of trace amoxicillin with satisfactory results.

关 键 词：共振瑞利散射 玫瑰精B 阿莫西林 

分 类 号：O657.39[理学—分析化学]