高效液相色谱法测定化妆品中乙内酰脲  

Determination of Hydantoin in Cosmetics by High Performance Liquid Chromatography

作　　者：彭莺[1] 吴玉銮[1] 陈意光[1] 柯振华[1] 冼燕萍[1] 潘莉[1] 罗海英[1] 

机构地区：[1]广州市质量监督检测研究院,国家化妆品质量监督检验中心(广州),广东广州510110

出　　处：《分析科学学报》2011年第2期264-266,共3页Journal of Analytical Science

基　　金：广东省科技计划(No.2008B080600007);国家质量监督检验检疫总局科技计划项目(2007QK302)

摘　　要：建立了高效液相色谱-二极管阵列检测器(HPLC-PDA)测定化妆品中乙内酰脲的方法。取1.0g样品,加入10mL乙腈,均质、离心、上清液浓缩、水溶解后,用C18固相萃取小柱净化,用水定容。经C18液相色谱柱分离,以甲醇和水为流动相,梯度洗脱,检测波长为210nm。在1～20mg/L范围内,色谱峰面积与乙内酰脲的质量浓度线性关系良好,相关系数为0.999 8,平均回收率为85.6%～95.5%,相对标准偏差4.1%～5.2%,方法检出限为0.08mg/kg。该法被成功用于水剂化妆品和霜类化妆品中乙内酰脲的测定。A high performance liquid chromatographic method was established for the determination of hydantoin in cosmetics.The mobile phase was methanol-water with gradient elution,the flow rate was 1.0 mL/min,the column temperature was 40℃ and the detection wavelength was 210 nm.The limit of quantification was 0.8 mg/kg.The correlation coefficient of linear calibration curve was above 0.9998 within the hydantoin concentration range of 1～25 mg/L.The average recoveries were from 85.6% to 95.5%.with the relative standard deviations from 4.1% to 5.2%.Aqueous and cream cosmetics were successfully analyzed by the proposed method with satisfactory results.

关 键 词：高效液相色谱法 乙内酰脲 化妆品 

分 类 号：O657.72[理学—分析化学]