低剂量氢醌对TK6淋巴母细胞生物学性状及miR-221表达影响  

Influences of biological characteristics and the expression of miR-221 induce by low-level hydroquinone exposure in TK6 lymphoblastoid cells

作　　者：刘林华[1] 梁小虎[1] 凌晓璇[1] 唐焕文[1] 

机构地区：[1]广东医学院公共卫生学院,广东东莞523808

出　　处：《中国职业医学》2011年第2期102-105,共4页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30972500);广东省自然科学基金项目(7301507);广东医学院青年科学基金资助项目(Q2009004)

摘　　要：目的探讨低剂量氢醌(HQ)对TK6淋巴母细胞的生物学性状及小分子非编码RNA(microRNA)-221(miR-221)表达的影响。方法磷酸盐缓冲液(PBS)溶解HQ,以PBS处理组为对照组,分别以2.5、5.0和10.0μmol/L HQ染毒TK6细胞为处理组。应用CCK-8试剂盒检测细胞活力和细胞增殖,通过磷脂结合蛋白(Annexin V)/碘化丙啶(PI)标记的流式细胞技术检测细胞凋亡,用实时荧光定量-聚合酶链反应(qRT-PCR)检测miR-221的表达改变。结果细胞增殖以48 h最为明显,2.5、5.0和10.0μmol/L组细胞增殖指数分别为1.33(P<0.05)、1.14(P<0.05)和1.12(P<0.05);miR-221表达改变以72 h最为明显,2.5、5.0、10.0μmol/L HQ组细胞miR-221表达量分别抑制了0.31倍(P<0.05)、0.39倍(P<0.05)和0.33倍(P<0.05)。结论低剂量HQ能抑制TK6细胞凋亡和miR-221的表达,促进细胞增殖。Objective To explore the influences of biological characteristics and the expression of small non-coding RNA（miR-221） induced by low-level hydroquinone（HQ） exposure in TK6 lymphoblastoid cells.Methods HQ dissolved in PBS buffer at the concentrations of 2.5,5.0 and 10.0μmol/L were respectively given to TK6 cells,and cells treated with PBS only were as the control.Cell viability and proliferation were detected with CCK-8 assay kit,the cell apoptosis assay was attained by Annexin V/PI apoptosis assay kit,while miR-221 expression was defined by reverse transcription real-time RT-PCR assay.Results Obvious alternation of cell growth occurred at 48 hours post HQ treatment,the proliferation indexes for 2.5,5.0 and 10.0 μmol/L HQ were 1.33（P0.05）,1.14（P0.05） and 1.12（P0.05）,respectively.The expression of miR-221 were inhibited by 0.31-（P0.05）,0.39-（P0.05） and 0.33-fold（P0.05） in the groups of 2.5,5.0 and 10.0 μmol/L HQ,respectively at 72 hours post treatment.Conclusion Low-level HQ could inhibit TK6cell apoptosis and miR-221 expression,while promote cell growth.

关 键 词：氢醌 TK6细胞 microRNA 细胞凋亡 细胞增殖 

分 类 号：R994.6[医药卫生—毒理学]