毒死蜱对雄性大鼠生精功能及相关激素水平影响  

Effect of chlorpyrifos on spermatogenesis function and sex hormone levels of male rats

作　　者：李相鑫[1] 赛林霖[1] 谢琳[1] 刘衍忠[1] 于功昌[1] 刘毅[1] 

机构地区：[1]山东省医学科学院山东省职业卫生与职业病防治研究院,山东济南250002

出　　处：《中国职业医学》2011年第2期136-138,共3页China Occupational Medicine

摘　　要：目的探讨毒死蜱对雄性大鼠生精功能和血清激素水平的影响。方法选择21 d龄无特定病原体级雄性W ister大鼠随机分成3个实验组和1个对照组,每组10只。实验组灌胃给予毒死蜱,剂量为2.7、5.4、12.8 mg·kg-1·d-1;对照组给予等量蒸馏水稀释的吐温80溶液。染毒90 d后,测定各组大鼠睾丸、附睾和前列腺-精囊腺等脏器系数,检测附睾尾精子数量、精子活动率和精子畸形率以及血清中性激素水平。结果高、中剂量组精子数量减少、精子畸形率升高、精子活力显著降低(P<0.05)。高、中剂量组血清睾酮(T)水平降低,促卵泡刺激素(FSH)升高(P<0.05)。不同组别血清中黄体生成素(LH)及雌二醇(E2)水平比较,差异无统计学意义(P>0.05)。结论毒死蜱对雄性大鼠有明显的生殖毒性,可影响雄性大鼠生精功能和血清中性激素水平。Objective To explore the effects of chlorpyrifos on spermatogenesis function and sex hormone levels of male rats.Methods 40 male Wistar rats,clean degree,were divided into four groups,the control（0 mg·kg-1·d-1）,low（2.7 mg·kg-1·d-1）,moderate（5.4 mg·kg-1·d-1）and high dose groups（12.8 mg·kg-1·d-1）.The rats were treated with chlorpyrifos by gavage,once a day for 90 consecutive days.Simultaneously,the controls were treated with equivalent volume of distilled water and tween-80.Then,the testis,epididymides and prostates-seminal vesicles were immediately excised and weighed,and the viscera coefficients were calculated.The number and motility of sperms in the tail of epididymis,the sex hormone levels were tested.Results In the moderate and high-dose groups,the numbers and motilities of sperms were significantly decreased,while the sperm abnormality rates were increased（P0.05）,serum testosterone levels were markedly decreased while serum FSH levels were increased（P0.05）.There were no significant differences between different groups of serum luteinizing hormone and estrogen.Conclusion Chlorpyrifos can produce reproductive toxicity in male rats,affecting their spermatogenesis function and sex hormone levels.

关 键 词：毒死蜱 大鼠 生精功能 性激素 

分 类 号：R139.3[医药卫生—劳动卫生] R996[医药卫生—公共卫生与预防医学]