首次实验室证实北京平谷地区恙虫病东方体暴发流行  

Laboratory confirmation of scrub typhus outbreak in Pinggu district,Beijing

作　　者：付秀萍[1] 刘玉英[2] 张宝华 王景泉[4] 张景山[1] 贺金荣[1] 张守印[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所立克次体室,北京102206 [2]北京平谷区医院 [3]北京平谷区中医院 [4]北京丰台铁营医院

出　　处：《中国媒介生物学及控制杂志》2011年第2期137-140,共4页Chinese Journal of Vector Biology and Control

基　　金：国家"十一五"科技重大专项课题(2008ZX10004-001)~~

摘　　要：目的了解北京市平谷地区是否存在恙虫病东方体感染。方法采用巢式聚合酶链反应(nPCR),对平谷地区采集的30份临床标本进行恙虫病东方体热休克蛋白(groEL)基因和56×103蛋白扩增并测序分析。采用间接免疫荧光试验(IFA)对血清标本恙虫病东方体特异抗体检测。结果采用PCR共检测血液标本30份,均阳性(100%);用IFA检测28份患者血清抗体,25份阳性(89.3%);长片段PCR扩增,3份标本扩增阳性,阳性结果测序比对所有核苷酸序列相同,与Kawasaki的同源性为96%。结论首次从分子流行病学和血清流行病学的角度证实北京平谷地区存在恙虫病东方体。Objective To understand the status of Orientia tsutsugamushi infection in Pinggu district,Beijing.Methods Nested-PCR（nPCR） was employed to amplify and sequence the groEL gene and 56×103 protein of O.tsutsugamushi from 30 samples collected in the district,with indirect immunofluorescence assay（IFA） used for the detection of the specific antibody to O.tsutsugamushi in the serum samples.Results There was a positive rate of 100% in terms of the gene and protein in the 30 blood samples tested with PCR,and 25 of the 28 serum samples that were tested by indirect immunofluorescence assay were found to be positive for the antibody,accounting for 89.3%.There were 3 samples that were positive with long fragment PCR amplification and DNA sequencing revealed that the sequences from all the samples were identical with a homology of 96% with Kawasaki type.Conclusion The existence of scrub typhus in Pinggu district of Beijing was for the first time confirmed from the perspective of molecular epidemiology and seroepidemiology.Our study indicates that physicians need pay attention to scrub typhus in febrile patients living in northern China where scrub typhus was not considered present in the past.

关 键 词：恙虫病 巢式聚合酶链反应 间接免疫荧光试验 

分 类 号：R376.2[医药卫生—病原生物学]