牙釉质基因鉴定山羊性别的研究  

Sex Identification in Goat by Amplification of Amelogenin (AMEL) Gene

作　　者：唐纪伟[1,2] 张志国 高庆华[2] 沈波[1,2] 武延风[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [3]大连金弘基种畜有限公司,辽宁大连116600

出　　处：《中国畜牧兽医》2011年第4期162-165,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30760170);国家科技支撑计划课题子课题(2008BADB2B05-1-3)

摘　　要：根据牙釉质(amelogenin,AMEL)同源基因在山羊X、Y染色体上存在不同程度碱基缺失的特点,设计1对引物,对山羊血液基因组DNA混合样进行PCR扩增,将PCR产物纯化、克隆并测序;然后对46个山羊血液DNA样本(♀:22个,♂:24个)进行性别鉴定。结果显示,雌性山羊样品经扩增只产生一条非特异性条带,雄性山羊样品产生一条非特异性条带和一条特异性条带;非特异性片段长度为349 bp,特异性片段为289 bp;46个山羊血液DNA样本鉴定结果与实际性别对比,雌性准确率为100%(22/22),雄性准确率为100%(24/24)。Based on the characteristic of the hircine AMEL-X and AMEL-Y gene with various degree deletions,a pair of primers were utilized to establish the process of sex identification by PCR and agarose gel electrophoresis with mixed samples of the hircine blood genomic DNA,and the PCR produced was purified,cloned and sequenced,then sex identification to the 46 hircine blood samples（♀：24,♂：22）.The results showed that the male hircine sample produces a non-specificity band and a specificity band,while the female hircine sample produces one band.The non-specificity fragment 349 bp,the specificity fragment 289 bp.By comparison,the results of the 46 hircine blood DNA samples sex identification with the hircine actual sex,the female accuracy 100%（22/22）,the male accuracy 100%（24/24）.

关 键 词：牙釉质基因 山羊 PCR 性别鉴定 

分 类 号：Q344.2[生物学—遗传学]