Trizol与RNA Fast1000试剂盒提取外周抗凝血总RNA的方法比较  

作　　者：武荣[1] 张俊涛[1] 赵霞[2] 程凯[1] 司海东[1] 赵虹[1] 

机构地区：[1]山西医科大学生物化学教研室,太原030001 [2]山西省疾病预防控制中心

出　　处：《中国医疗前沿》2011年第5期60-61,共2页China Healthcare Innovation

摘　　要：目的比较传统Trizol法和试剂盒法提取血液中总RNA的效果。方法我们将100份人体新鲜血液标本随机分为2组,每组各50份,分别用Trizol法和RNA Fast1000试剂盒法两种方法从外周血中提取RNA测定其纯度和完整性,通过两独立样本的t检验统计分析2组OD值比值数据的差异性。结果试剂盒提取的总RNA的纯度(1.904±0.15)优于传统的Trizol方法(1.727±0.42)。结论与Trizol法相比,试剂盒法具有简便、快速、高纯度等特点,且提取的RNA样品质量较高,可直接用于RT-PCR合成、Northern杂交等分子生物学操作。Objective To compare the effect which traditional Trizol method and kit method has been used to extract the total RNA from blood.Methods we put 100 pieces of fresh blood samples from human randomly and divided into two groups,each group,50 pieces.One group was extracted RNA with Trizol method from the peripheral blood and measured its purity and integrity.Another group was extracted RNA with RNA Fast1000 kit method from the peripheral blood and measured its purity and integrity.At last we analysis the differences of the OD from two groups through two independent sample t-test statistical.Results The total RNA purity which was extracted with kits method is better.Conclusion To compared with Trizol method,the kit method is simple and convenient,rapid,high purity,the RNA sample quality extracted with kit method is higher.The kit method can be applied to molecular biology operation directly,for example： RT-PCR synthesis,Northern hybridization.

关 键 词：外周血液 总RNA 提取 

分 类 号：R446.6[医药卫生—诊断学]