苯酚与亚硝酸根相互作用分光光度法测定亚硝酸根  

Determination of nitrite with Phenol-nitrite Reaction by Spectrophometry

作　　者：郎燕玲[1] 林仁权[1] 王雪君[1] 

机构地区：[1]浙江省杭州市拱墅区疾病预防控制和妇幼保健中心,杭州310014

出　　处：《中国卫生检验杂志》2011年第3期597-597,600,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:测定肉与肉制品中微量亚硝酸根含量。方法:在盐酸介质中,苯酚与亚硝酸根相互作用,生成亚硝基苯酚,该溶液在最大吸收波长为350 nm。据此建立测定亚硝酸根的方法。结果:线性范围为0 mg/L～10 mg/L,检出限为0.29 mg/L。结论:方法应用于肉与肉制品中微量亚硝酸根含量测定,结果满意。Objective：For the determination of nitrite in meat and meat products.Methods：A new ultraviolet spectrophometric method for the determination of nitrite was proposed,which is based on the phenazone-nitrite reaction,producting nitroso-phenol,in the Hydrochloric Acid.the nitrite content was measured at 350 nm.Results：Nitrite in the linear concentration range of 0 mg/L～10 mg/L was determined with a detection limit of 0.29 mg/L.Conclusion：The method has been applied to the determination of nitrite in meat and meat products with satisfactory results.

关 键 词：紫外分光光度法 苯酚 亚硝酸根 

分 类 号：O657.32[理学—分析化学]