人参皂苷Rg3对人肺腺癌细胞株A549/DDP抑制转移及逆转耐药作用的研究  

Studies of 20(R)-ginsenoside Rg3 on restraining metastasis and reversing multidrug resistance in A549/DDP cell line

作　　者：王艳[1] 刘静蕾[1] 刘莉[1] 赵红丽[1] 李欣[1] 杨茂鹏[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国卫生检验杂志》2011年第3期609-611,614,共4页Chinese Journal of Health Laboratory Technology

基　　金：黑龙江省自然科学基金资助课题(D200849)

摘　　要：目的:探讨人参皂苷Rg3抑制人肺腺癌细胞株A549/DDP的浸润及转移和逆转其耐药作用及机制。方法:应用MTT法检测Rg3对A549/DDP细胞逆转耐药的作用;用细胞膜流动性实验检测Rg3对A549/DDP细胞膜流动性的改变用穿膜实验检测Rg3对A549/DDP细胞侵袭和转移的影响;用Western blotting实验检测Rg3对转移和耐药相关蛋白表达的影响。结果:Rg3作用后,A549/DDP细胞对顺铂的耐药性下降I,C50明显降低(P<0.05),细胞膜的流动性明显降低(P<0.05),细胞的穿膜能力明显降低(P<0.05),Rg3作用后,nm23及caspase-3的表达明显上调,LRP的表达无明显改变。结论:Rg3可通过上调nm23的表达抑制A549/DDP细胞的侵袭和转移能力,还可通过上调caspas-3的表达,降低细胞膜的流动性达到逆转耐药的作用。Objective：To observe and study the mechanism of restraining the metastasis and reversing multidrug resistance（MDR） of A549/DDP cell line by 20（R）-ginsenoside Rg3.Methods：MTT was applied to determine the chemosensitivity of A549/DDP to cisplatin,cell membrane lipid fluidity was determined to observe the Rg3 effect to cell,cell invasion assay was applied to determine the invasion change of Rg3 to cell,the protein expression levels of nm23,caspase-3 and LRP were determined by Western blotting analyses.Results：Rg3 could make the IC50 and drug-resistant index of cisplatin decrease;cell membrane lipid fluidity was decreased;cell invasion ability decreased;expression of nm23 and caspase-3 were upregulated,and LRP expression had no obvious change.Conclusion：Ginsenoside Rg3 can restrain A549/DDP cell invasion and metastasis,and it may have relationship with the upregulation of nm23;Rg3 can enhance the A549/DDP cell chemosensitivity to cisplatin,and it may have relationship with the upregulation of caspase-3 and the cell membrane lipid fluidity decreased.

关 键 词：人参皂苷RG3 转移 多药耐药 

分 类 号：R965[医药卫生—药理学]