吸毒人员外周血CD4^+ CD25^+ Foxp3调节性T细胞表达  

Expression of CD4^+CD25^+ Foxp3 regulatory T cells in the blood of drug abuse population

作　　者：庞惠勇 葛恒明 陈晓芹[1] 刘小林[2] 李忠典 张振宇[1] 张健[1] 

机构地区：[1]江苏省东海县疾病预防控制中心,江苏东海222300 [2]江苏省连云港市第二人民医院,江苏连云港222023

出　　处：《中国卫生检验杂志》2011年第3期664-665,667,共3页Chinese Journal of Health Laboratory Technology

基　　金：江苏连云港市医药卫生科研课题(09021)

摘　　要：目的:探讨外周血CD4+CD25+Foxp3调节性T细胞与吸毒人员机体免疫的关系。方法:采集114名吸毒人员外周血,根据不同吸毒方式和吸毒年限进行分组,应用流式细胞仪检测外周血中CD4+CD25+Foxp3调节性T细胞表达。结果:不同吸毒方式的吸毒人员外周血CD4+CD25+Foxp3调节性T细胞表达,以口吸、静脉注射、混合吸顺序递减(49.07%±14.88%、34.96%±13.41%、26.72%±8.49%),各组间两两比较,差别有统计学意义(P<0.01);同一吸毒方式不同吸毒年限的吸毒人员CD4+CD25+Foxp3调节性T细胞表达相比较,≤10年的口吸组(51.79%±10.44%)较>10年口吸组(37.14%±12.29%)明显降低(P<0.01),而混合吸毒组明显升高(27.06%±8.99%到35.47%±11.02%,P<0.05),静脉注射组变化不明显(P>0.05)。结论:通过检测吸毒人员外周血CD4+CD25+Foxp3调节性T细胞的表达,反映了不同吸毒方式和吸毒年限的吸毒人员机体免疫功能损伤程度,不仅可作为一项准确、可靠监测指标,也将为吸毒人员的免疫功能的检测提供新的思路。Objective：This study was designed to investigate the effect of drug abuse on human immune function by examining the blood CD4＋CD25＋ Foxp3 regulatory T cell expression.Methods：Blood samples were collected in 114 different drug taken route and period people.Flow cytometry was employed to examine the expression of CD4＋CD25＋ Foxp3 regulatory T cells.Results：The expression of CD4＋CD25＋ Foxp3 regulatory T cells was different among different taken route groups（Oral,49.07%±14.88%intravenous injection,34.96%±13.41%mixed routes,26.72%±8.49%）.There were significant differences（P0.01） between any of the above two groups.We also examined the effect of drug taken period on the expression of CD4＋CD25＋ Foxp3 regulatory T cells.For oral taken people,the expression was much lower in the people with taken period longer than 10 years（37.14%±12.29%） compared with those people with shorter drug taken period（≤10 years）（51.79%±10.44%,P0.01）.For mixed taken route patients,however,the expression increased from 27.06%±8.99% in people with ≤10-year drug taken period to 35.47%±11.02% in people with 10-year drug taken period（P0.01）.There was no significant difference in the intravenous injection group（P0.05）.Conclusion：By examining the blood CD4＋CD25＋ Foxp3 regulatory T cell expression in the drug abuse population,it was found that different drug taken routes and periods may induce different extents of injury to the body immune function.Our results provide not only an accurate,reliable monitoring index,but also a new approach to examine the immune function in drug abuse population.

关 键 词：CD4+CD25+Foxp3调节性T细胞 吸毒方式 吸毒年限 

分 类 号：R996[医药卫生—毒理学]