甘肃省部分地区病毒性脑炎标本的实验室检测  

Laboratory testing of specimens from patients with viral encephalitis from some regions of Gansu

作　　者：蒋建祥[1] 高续明[1] 于德山[1] 

机构地区：[1]甘肃省疾病预防控制中心,兰州730000

出　　处：《中国卫生检验杂志》2011年第3期685-686,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:初步了解甘肃省病毒性脑炎的病原种类及其分布特征。方法:用ELISA方法对2006年-2009年甘肃省5个地、州、市送检的209例临床诊断为病毒性脑炎患者的急性期血清和脑脊液标本,检测乙脑病毒IgM抗体,然后对乙脑IgM抗体阴性的所有血清、脑脊液标本检测其它6种常见病毒IgM抗体。结果:经血清学及脑脊液检测,209例患者中的154例(73.7%)检测出病毒特异性IgM抗体,构成顺序为乙脑病毒(16.7%)、呼吸道合胞病毒(15.8%)、腮腺炎病毒(13.4%)柯萨奇病毒(9.6%)、埃可病毒(9.1%)、单纯疱疹病毒(8.1%)、水痘-带状疱疹病毒(1.0%)。结论:乙脑病毒及呼吸道合胞病毒是甘肃省病毒性脑炎的主要病原体,腮腺炎病毒次之,柯萨奇病毒、埃可病毒、单纯疱疹病毒也是重要的抗原。Objective：To understand the viral etiology of viral encephalitis in Gansu by decting IgM antibody in the clinical samples of patients from some regions of Gansu by enzyme-linked immunosorbent assay.Methods：Serum and cerebrospinal fluid samples of 209 patients with viral encephalitis or meningitis were collected from five regions of Gansu and were stored at-70℃.Enzyme-linked immunosorbent assays were used for detection of IgM antibody to Japanese encephalitis virus,coxsackievirus,echovirus,herps simplex virus,mumps virus,varicella-zoster virus,respiratory syncytial virus.Results：IgM antibody was detected in 154 of 209（73.7%） cases,the most common pathogen was Japanese encephalitis virus（16.7%）,followed by respiratory syncytial virus（15.8%）,Mumps（13.4%）,coxsackievirus（9.6%）,echovirus（9.1%）, herps simplex virus（8.1%）,varicella——zoster virus（1.0%）.Conclusion：According to the study,Japanese encephalitis was the important encephalitis in Gansu,Respiratory syncytial virus was another important pathogen.Mumps and coxsackievirus were also important pathogens.

关 键 词：脑炎 病毒性 病原 

分 类 号：R515.2[医药卫生—内科学]