LC-MS/MS法快速高效检测尿液中尼古丁及其9种代谢物  

A Rapid LC-MS/MS Method for Determination of Nicotine and Its 9 Metabolites in Urine

作　　者：周宛虹[1] 王晔[1] 余苓[1] 金永明[1] 郑赛晶[1] 

机构地区：[1]上海烟草集团有限责任公司,上海200082

出　　处：《化学学报》2011年第7期803-809,共7页Acta Chimica Sinica

摘　　要：以氘代尼古丁、氘代可的宁、3-OH-氘代可的宁、氘代尼古丁葡萄糖醛酸复合物、氘代可的宁葡萄糖醛酸复合物、3-OH-氘代可的宁葡萄糖醛酸复合物作内标,在LC-MS/MS的大气压化学电离(APCI)离子化模式下,建立了快速检测吸烟者尿液中尼古丁及其9种代谢物的方法,并对19个实际样品进行了测试和判别分析(DA).实验前处理简单,色谱运行时间仅为3.8 min.结果表明,尼古丁及其代谢物的精密度在0.5%～5.5%之间,回收率在94%～109%之间,线性相关系数均大于0.995.DA分析充分区别开了不同量的吸烟者,进一步的相关性分析表明吸烟者24 h尿液中的尼古丁及其9种代谢物含量与卷烟抽吸量的分析因子之间均有较强正相关性.A fast, highly efficient method for the detection of nicotine and its nine metabolites in urine was established in this paper. The quantification was achieved by using d3-nicotine, d3-cotinine, d3-3-OH-cotinine, d3-nicotine glucuronic acid, d3-cotinine glucuronic acid and d3-3-OH-cotinine glucuronic acid as internal standards under atmosphere pressure chemical ionization （APCI） condition of LC-MS/MS. Nineteen samples were quantified and then analyzed by discriminant analysis （DA）. The pretreatment of this method was simple, time-saving and all compounds were eluted within 3.8 min. The relative standard deviation （RSD%） of nicotine and its nine metabolites were between 0.5% and 5.5% with the recovery rate ranged from 94% to 109%. The linear correlations were no less than 0.995. Smokers of different levels were significantly distinguished using DA and a strong positive correlation was found between the value of nicotine and its 9 metabolites in 24 h urine and smoke amount.

关 键 词：LC-MS/MS 快速检测 尿液 尼古丁 代谢物 判别分析 

分 类 号：R446.12[医药卫生—诊断学]