鲍氏针层孔菌菌丝体多糖脱蛋白方法研究  

Study on Methods of Deproteinization from Phellinus baumii Polysaccharide

作　　者：谢丽源[1] 张勇[2] 彭卫红[1] 甘炳成[1] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066 [2]电子科技大学生命科学与技术学院,四川成都610054

出　　处：《中药材》2011年第2期293-295,共3页Journal of Chinese Medicinal Materials

基　　金：四川省青年基金项目(2008ZQ026-072);四川省科技支撑计划项目(2008FZ0157)

摘　　要：目的:对多糖提取液中的蛋白质去除方法和条件进行了优化,比较不同方法对蛋白质去除的影响。方法:采用Sevag法与三氯乙酸(TCA)法去除鲍氏针层孔菌多糖中的游离蛋白质。结果:TCA去除蛋白质的效果优于Sevag法,TCA最佳脱蛋白条件为:5%三氯乙酸用量,处理3次,反应时间30min;在此条件下,蛋白质去除率为82%,多糖损失率为10.8%。结论:TCA法为最佳的鲍氏针层孔菌脱蛋白方法。Objective：To study and compare different methods and influencing factors of deproteinization from Phellinus baumii polysaccharide.Methods：Sevag method and TCA method were used to remove protein from crude polysaccharide of Phellinus baumii,respectively.Results：The effect of TCA method on removing protein was superior to that of Sevag method,and the optimum conditions of removing protein with TCA were as follows：30min treating time,5% trichloroacetic acid,3 times treatemt.Under these conditions the rate of protein-removed was 82% and the rate of polysaccharide-lost was 10.8%.Conclusion：TCA method is the optimal mean for deproteinization from Phellinus baumii polysaccharide.

关 键 词：鲍氏针层孔菌 Sevag法 TCA法 多糖 蛋白质去除率 多糖损失率 

分 类 号：R284.2[医药卫生—中药学]