人隐孢子虫IbA19G2基因亚型蒙古沙鼠感染模型的建立  

Laboratory Model for Cryptosporidium hominis IbA19G2 Subtype in Mongolian Gerbils

作　　者：王强[1] 杨楠[1] 菅复春[1] 王荣军[1] 宁长申[1] 张龙现[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《寄生虫与医学昆虫学报》2011年第1期1-5,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家重大传染病专项重要寄生虫病检测和监测技术研究（2008ZX10004-011）;河南省重大公益科研项目（81100912300）

摘　　要：人隐孢子虫Cryptosporidium hominis是人类的主要感染虫株,目前已获取且传代保存的国内分离株较少,在生物学特性研究也未见报道.在本试验自感染前第10天起至排卵囊结束后14天,采用胃管灌服地塞米松（0.75 mg/（只·2天））抑制蒙古沙鼠免疫力,对国内C.hominis Ib亚型分离株生物学特性进行研究.结果显示,感染后第3天,感染组有卵囊排出,感染后第6天出现高峰期,此后逐渐下降直至感染后第10或11天排卵囊结束.感染前后基于18S rRNA和gp60基因分析,表明遗传学无差异.建立了持续稳定的C.hominis Ib亚型分离株啮齿动物模型,为C.hominis卵囊扩增和进一步研究其生物学特性等提供了重要的参考依据.Researches have shown that Cryptosporidium hominis is one of the main Cryptosporidium parasites for human cryptosporidiosis in China. However, only a few isolates of C. hominis were obtained and serially sub-cultivated in laboratory up to date, so the biological characteristics of C. hominis remains unreported in our country. In the present work, Mongolian gerbils （Meiiones unguiculataus） were orally inoculated with 0.75 mg of dexamethasone/animal by using gastric tube drench every two days from the 10th day before oocyst ingestion. The results showed that the gerbils started to shed oocysts in the third day after inoculation （DAI） , the number of shed oocysts increased obviously, reached the peak value at the 6th DAI, and then dropped gradually until 10th or llth DAI. The 18S rRNA and gp60 gene analysis performed before and after inoculation revealed no genetic difference of C. hominis oocysts. A stable rodent model of C. hominis Ib subtype infection has been successfully established, which may be an important value for the further study on the biological characteristics of C. hominis.

关 键 词：人隐孢子虫 蒙古沙鼠 免疫抑制 感染模型 

分 类 号：Q78[生物学—分子生物学]