弓形虫速殖子入侵对宿主细胞窖蛋白-1的表达变化  

Study on the Change of Caveolin-1 During the Invasion of Toxoplasma gondii

作　　者：覃珊珊[1] 汪琦[1] 朱家勇[1] 江钢锋[1] 李娟兰[1] 

机构地区：[1]广东药学院基础学院寄生虫学教研室,广州510006

出　　处：《寄生虫与医学昆虫学报》2011年第1期11-14,共4页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：胞膜窖Caveolae,又称细胞膜穴样凹陷,是细胞表面特异性的内陷微区,Caveolin-1是其的标记蛋白.Caveolae结构和caveolin蛋白在多种细胞生理功能方面如细胞信号转导、胆固醇转运、内吞和肿瘤抑制等发挥着重要作用.在动脉硬化、肌营养不良和Alzheimer＇S综合征等多种疾病中均发现存在caveolae和caveolin的异常.作者根据GenBank中caveolin-1的基因序列设计引物,RT-PCR和实时荧光定量PCR检测不同数量的弓形虫速殖子入侵Hela细胞10、30和60 min时,caveolin-1的表达变化.结果显示弓形虫入侵宿主细胞后caveolin-1的表达与虫体的浓度呈正相关,即caveolin-1的表达量随虫体浓度的增加而升高,并随着感染时间的延长而呈增加趋势.推测宿主细胞可能参与了弓形虫的主动入侵过程,研究结果将为深入研究弓形虫致病机制奠定基础.Caveolae is a specific invagination mini-district, and caveolin-1 is a marker protein of caveolae. Our work focus on the expression change of caveolin-1 during the invasive process for the further study on the control and treatment of Toxoplasmosis. Based on gene sequence of caveolin-1 in GenBank, we designed primers for RT-PCR and real-time PCR to detect the changes of the expression of caveolin-1 at 10 min, 30 min and 60 rain after invasion of Toxoplasma gondii into the host ceils. The results showed that the expression of caveolin-1 was positive correlation with the concentration of the parasites. With the increase of the parasites, the expression of caveolin-1 increased, and also with the increase of infection times, it showed an increasing tendency. It was concluded that caveolin 1 may participate the process of T. gondii initiative invasion.

关 键 词：刚地弓形虫 窖蛋白-1 实时荧光定量PCR 

分 类 号：Q954.434[生物学—动物学]