黄粉虫抗菌肽促K562细胞凋亡作用及其机制的研究  

Effect of the Antimicrobial Peptide of Tenebrio molitor L. on Mitochondrial Membrane Potential and Caspase-3 of K562 Cells

作　　者：樊宏英[1] 刘颜岗[2] 赵瑞君[1] 乔丛静[1] 申金雁[1] 

机构地区：[1]山西医科大学寄生虫学教研室,太原030001 [2]秦皇岛市第三医院,河北秦皇岛066000

出　　处：《寄生虫与医学昆虫学报》2011年第1期38-41,共4页Acta Parasitologica et Medica Entomologica Sinica

基　　金：山西省高校科技研究开发项目（200613011）;山西省科技攻关项目（2006031087-04）;太原市科学技术发展计划项目（081049）

摘　　要：为观察黄粉虫抗菌肽对K562细胞线粒体膜电位及K562细胞凋亡蛋白半胱天冬氨酸蛋白酶-3（caspase-3）的影响,进而探讨黄粉虫抗菌肽否是影响线粒体膜电位以及是否可以引起K562细胞凋亡,本文首先采用荧光探针罗丹明123 标记K562细胞,在激光扫描共聚焦显微镜下观察K562细胞中罗丹明123荧光强度,以细胞内荧光强度表示线粒体膜电位大小;其次用caspase-3检测试剂盒和酶标仪测定K562细胞caspase 3的含量.实验结果显示,抗菌肽组的K562细胞荧光强度显著低于对照组（t=5.635,P〈0.05）,说明黄粉虫抗菌肽可使K562细胞线粒体膜电位降低;抗菌肽组caspase 3的含量显著高于对照组（t=43.289,P〈0.05）,说明黄粉虫抗菌肽可以诱导K562细胞发生凋亡.结果表明,黄粉虫抗菌肽可以诱导K562细胞发生凋亡,作用机制是通过降低K562细胞线粒体膜电位,扰乱其生理功能,促使其凋亡.In order to observe the effect of the antibacterial peptide of Tenebrio molitor on the mitochondrial membrane potential and apoptosis protein caspase-3 of K562 cells, laser scanning confocal microscopy （LSCM） was used to observe the fluorescence intensity of the rhodamine 123 in K562 cells, and the enzyme activity of caspase-3 in K562 cells was detected with Caspase-3 kit and immunosorbent assay. The intracellular fluorescence intensity indicated the mitochondrial membrane potential, and the rhodamine 123 served as a fluorescence probe to label the K562. The results showed that the fluorescence intensity of the antibacterial peptide group is significantly lower than that of control group （ t = 5. 635, P 〈 0. 05 ） , implying that the antibacterial peptide can reduce the mitochondrial membrane potential of K562 cells. The activity of caspase-3 in antibacterial peptide group is significantly higher than that in control group （ t = 43. 289, P 〈 0. 05 ） , implying that the antibacterial peptide can promote the apoptosis of K562 cells.

关 键 词：抗菌肽 线粒体膜电位 CASPASE-3 细胞凋亡 

分 类 号：Q255[生物学—细胞生物学]