姜黄素对哮喘大鼠IL-5、Eotaxin 水平及NF-κB表达的影响  

Effects of curcumin on IL-5 level and expression of NF-κB and eotaxin in asthmatic rats

作　　者：黄建达[1] 应骏[1] 顾铜杰[1] 

机构地区：[1]宁波市第二医院呼吸科,315010

出　　处：《浙江医学》2011年第3期334-336,340,共4页Zhejiang Medical Journal

摘　　要：目的 探讨姜黄素对支气管哮喘（简称哮喘）大鼠血清、支气管肺泡灌洗液（BALF）中IL-5 及肺组织中 Eotaxin、NF-κB表达的影响.方法 将36只大鼠随机分为正常对照组、哮喘组、姜黄素组,用卵白蛋白（OVA）制备哮喘大鼠模型,正常对照组用0.9%氯化钠溶液代替OVA进行致敏和激发.以ELISA法检测各组大鼠血清及BALF中IL-5的浓度以及计数BALF中嗜酸细胞（EOS）的绝对值及百分比,同时以免疫组化方法检测Eotaxin、NF-κB等在肺组织中的表达.结果 血清及BALF中IL-5浓度的改变与NF-κBp65、Eotaxin蛋白表达的改变：哮喘组及姜黄素组均显著高于正常对照组（P＜0.01）,姜黄素组显著低于哮喘组（P＜0.01）.肺组织细胞NF-κB p65的表达与EOS绝对计数、IL-5的表达及Eotaxin的表达均呈显著正相关（r=0.928、0.905、0.850,均P＜0.01）.结论 姜黄素能抑制NF-κB的活性,通过抑制NF-κB向核内转移与DNA的结合而减少IL-5及Eotaxin的合成,从而发挥抗哮喘作用.Objective To investigate the effects of curcumin on IL-5 levels in serum and bronchoalveolar lavage fluid （BALF）, and expression of NF- K B and eotaxin in lung tissue of asthmatic rats Methods Thirty six rats were randomly divided into three groups： normal control group （group A）, asthma group （group B）, curcumin treatment group （group C）. The asthma model was induced by ovalbumin （OVA） challenge. The concentrations of IL-5 in serum and BALF were measured by sandwich ELISA, EOS cells in BALF were counted,the expressions of NF- κB p65 and eotaxin were determined by immunehistochemistry techniques. Results The concentrations of IL-5 in serum and BALF of group B was significantly higher than those of group A and group C （P〈0.01）. Immunohistochemistry showed that the expression of NF-κB p65 of was in cytoplasm and nuclei of epithelial cells and inflammatory cells. The expression of NF-κB P65 in group B was significantly higher than those of group A and group C （P〈 0.01）. Immunohistochemistry showed that eotaxin was mainly in cytoplasm of the bronchus epithelial cells. The expression of Eotaxin in group B was significantly higher than those of group A and group C （P〈 0.01）. The expression of NF- κB p65 in lung tissue was positively correlated with EOC count, IL-5 level and eotaxin expression （r=0.928, 0.905, 0.850,all P〈 0.01 ）. Conclusion Curcumin can significantly down-regulate NF- κB and in turns inhibit IL-5 and eotaxin expression in asthmatic rats.

关 键 词：姜黄素 支气管哮喘 NF—κB EOTAXIN IL-5 

分 类 号：R562.25[医药卫生—呼吸系统]